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Identification of CD105 (endoglin)-positive stem-like cells in rhabdoid meningioma

  • Laboratory Investigation - Human/Animal Tissue
  • Published:
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Abstract

To investigate the tumor-initiating cells (TICs) in rhabdoid meningioma (RM), a population of CD105-positive cells isolated from a fresh RM surgical sample was analyzed for proliferative activity, self-renewal ability, tumorigenic ability, multilineage differentiation potential, as well as chromosomal aberrations. The results showed that isolated CD105-positive cells could be maintained for more than 50 generations in vitro. These cells exhibited increased proliferative activity and single-cell tumor sphere-formation ability compared with CD105-negative cells. In vivo experiments showed that CD105-positive cells possessed much greater potential to reconstitute the original human RM in nude mice as compared with CD105-negative cells. Phenotypically, CD105-positive cells shared some surface markers with mesenchymal progenitor cells (MPCs), but karyotype analysis showed chromosomal abnormalities characteristic of malignant meningioma, thus distinguishing them from supportive stroma-derived MPCs. In addition, in contrast to CD105-negative cells, CD105-positive cells could differentiate into adipocytes and osteocytes in response to specific induction agents. Finally, CD105-positive cells with stem-like features were also isolated from xenograft tumors. In conclusion, a population of CD105-positive TICs with some traits of MPCs was identified in RM and might provide a promising therapeutic target in management of malignant meningioma.

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Abbreviations

RM:

Rhabdoid meningioma

VIM:

Vimentin

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Acknowledgments

The authors thank Dr. Guoping Zhang for technique support in Flow cytometric analysis, Dr. Xuqun Tang for help in using the statistic software, and Dr. Keke Zhou for help in preparing the manuscript.

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Authors and Affiliations

  1. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China

    Dezhi Hu, Ying Mao & Liangfu Zhou

  2. Department of Neurology, The Tenth People’s Hospital, Tongji University, Shanghai, China

    Xiaomei Wang

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  1. Dezhi Hu
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  2. Xiaomei Wang
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  3. Ying Mao
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  4. Liangfu Zhou
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Corresponding author

Correspondence to Ying Mao.

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Dr. Dezhi Hu and Dr. Xiaomei Wang contributed equally to this work.

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Hu, D., Wang, X., Mao, Y. et al. Identification of CD105 (endoglin)-positive stem-like cells in rhabdoid meningioma. J Neurooncol 106, 505–517 (2012). https://doi.org/10.1007/s11060-011-0705-3

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  • DOI: https://doi.org/10.1007/s11060-011-0705-3

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