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Regulation of histone acetylation by NDRG2 in glioma cells

  • Laboratory Investigation - Human/Animal Tissue
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Abstract

NDRG2, a member of the N-Myc downstream-regulated gene family, was shown to be a putative tumor suppressor gene in glioblastoma and other cancers. Through a bioinformatic analysis, we found that NDRG2 protein contains an acyl carrier domain. In the current study, we therefore hypothesized that NDRG2 may play an important role in the regulation of histone acetylation. Treatment of U251 and U87 glioma cells with trichostatin A, an inhibitor of histone deacetylase, upregulated the expression of NDRG2 and acetylated forms of histones H3 and H4, reduced tumor cell viability and arrested the cell cycle at the G1/G0 phase. Overexpression of NDRG2 by transfecting glioma cells with adenovirus containing the NDRG2 gene upregulated the levels of acetylated forms of H3 and H4 whereas inhibition of NDRG2 expression by siRNA-mediated knockdown downregulated the level of histone acetylation. Furthermore, NDRG2 siRNA significantly reduced the level of histone acetylation induced by trichostatin A. Taken together, these data demonstrate that NDRG2 can regulate the level of histone acetylation to control glioma cell growth.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30672168).

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None.

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Authors and Affiliations

  1. Department of Neurology, Xijing Hospital, The Fourth Military Medical University, 15 Changle Xi Road, Xi’an, 710032, China

    Li Li, Ming Shi, Rui Miao, Li Wang & Yanchun Deng

  2. Department of Neurology, Xi’an North Hospital, 170 Changle Xi Road, Xi’an, 710032, China

    Xiaoqin Qin

  3. Department of Biochemistry and Molecular Biology and The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, 17 Changle Xi Road, Xi’an, 710032, China

    Xinping Liu & Libo Yao

Authors
  1. Li Li
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  2. Xiaoqin Qin
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  3. Ming Shi
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  5. Li Wang
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  6. Xinping Liu
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  8. Yanchun Deng
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Corresponding author

Correspondence to Yanchun Deng.

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Li Li and Xiaoqin Qin contributed equally to this work.

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Li, L., Qin, X., Shi, M. et al. Regulation of histone acetylation by NDRG2 in glioma cells. J Neurooncol 106, 485–492 (2012). https://doi.org/10.1007/s11060-011-0700-8

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  • DOI: https://doi.org/10.1007/s11060-011-0700-8

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