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Overexpression of septin 7 suppresses glioma cell growth

  • Laboratory Investigation - Human/Animal Tissue
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

Our previous study demonstrated that SEPT7 was downregulated at mRNA level in human gliomas. This study is to further examine the expression of SEPT7 in glioma samples and characterizes its role on cell cycle progression and growth of glioma cells. mRNA and protein expression of SEPT7 were detected by RT-PCR, immunohistochemical staining, and western blot analysis in human glioma specimens and normal brain tissues. A pcDNA3-SEPT7 expression plasmid was constructed and transfected into human glioblastoma cell line U251, and cell proliferation and apoptosis were examined. The growth of established U251 and TJ905 subcutaneous xenograft gliomas was measured in nude mice treated with pcDNA3-SEPT7 and U251 xenograft tumors treated with SEPT7 siRNA. SEPT7 expression is negatively correlated with the increase of glioma grade. Overexpression of SEPT7 is able to inhibit cell proliferation and arrest cell cycle progression in the G0/G1 phase both in vitro and in vivo. Knocking down further the already low endogenous expression of SEPT7 in U251 xenograft tumors with siRNA leads to faster tumor growth compared with control tumors. This study demonstrates that SEPT7 is involved in gliomagenesis and suppresses glioma cell growth.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Grant No: 30500523, Grant No: 30872985), the Program for New Century Excellent Talents in University (Grant No: NCET-07-0615), the Tianjin Education Commission (Grant No: 20070234), and the Tianjin Municipal Science and Technology Commission (Grant No: 06YFSZSF01100).

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Authors and Affiliations

  1. Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-Oncology, Tianjin Neurological Institute, 152 An-Shan Road, Tianjin, 300052, People’s Republic of China

    Zhi-fan Jia, Qiang Huang, Chun-sheng Kang, Wei-dong Yang, Guang-xiu Wang, Shi-zhu Yu & Pei-yu Pu

  2. Department of Neurology, Henry Ford Hospital, Detroit, MI, 48202, USA

    Hao Jiang

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  1. Zhi-fan Jia
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  2. Qiang Huang
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  3. Chun-sheng Kang
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  4. Wei-dong Yang
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  5. Guang-xiu Wang
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  6. Shi-zhu Yu
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  7. Hao Jiang
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  8. Pei-yu Pu
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Correspondence to Pei-yu Pu.

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Zhi-fan Jia and Qiang Huang contribute equally to this work.

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Jia, Zf., Huang, Q., Kang, Cs. et al. Overexpression of septin 7 suppresses glioma cell growth. J Neurooncol 98, 329–340 (2010). https://doi.org/10.1007/s11060-009-0092-1

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  • DOI: https://doi.org/10.1007/s11060-009-0092-1

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