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The cell-cycle kinetics of craniopharyngioma and its clinical significance

  • Clinical Study - Patient Study
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Abstract

Craniopharyngioma (CP) is a pathologically benign tumor with high incidence of recurrence and poor prognosis. DNA ploidy, S-phase fraction (SPF), and G2 phase/mitosis phase + S phase (G2/M + S) measured by flow cytometry (FCM) have been shown to correlate with cell cycle characteristics and clinical prognosis of other tumors. By use of FCM and terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) peroxidase, we compared DNA content, SPF, G2/M + S, necrosis and apoptosis in non-recurrent and recurrent tumor cells of CP from 63 cases including 32 adamantine epitheliomas (AEs) and 31 squamous papillary tumors (SPTs), and the ultrastructure of the CP cell was observed by transmission electron microscopy. Although no obvious differences between DNA content and necrosis and apoptosis rate were observed in subgroups of CPs, SPF and G2/M + S for recurrent tumors were statistically higher than those for recurrence-free tumors, and the recurrence rate of AE tumors is higher than that of SPT. Therefore, CP cells are diploid, and SPF and G2/M + S are related to recurrence of CP.

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Abbreviations

CP:

Craniopharyngioma

SPF:

S-phase fraction

G2/M + S:

G2 phase/mitosis phase + S phase

FCM:

Flow cytometry

TUNEL:

Terminal deoxynucleotide transferase-mediated dUTP nick end labeling

AE:

Adamantine epithelioma

SPT:

Squamous papillary tumor

MRI:

Magnetic resonance imaging

PI:

Propidium iodide

MIB-LI:

MIB labeling index

MCM6:

Minichromosome maintenance protein 6

DNA TopII alpha:

DNA topoisomerase II alpha

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Acknowledgments

This work was supported by grant No. 30872646 from the National Natural Science Foundation of China and the China Medical Board of New York. The authors thank all cooperating neurosurgeons who gave us permission to recruit and interview their patients and the pathologists who helped us in pathohistological diagnosis and classification of the subtypes of the craniopharyngiomas.

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Authors and Affiliations

  1. Department of Neurosurgery, West China Hospital, Sichuan University, 37# Guoxuexiang Street, 610041, Chengdu, People’s Republic of China

    Jianguo Xu, Chao You, Liangxue Zhou, Qiang Li & Peizhi Zhou

  2. Department of Pathology, West China Hospital, Sichuan University, 37# Guoxuexiang Street, 610041, Chengdu, People’s Republic of China

    Ni Chen

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  1. Jianguo Xu
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  2. Chao You
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  3. Liangxue Zhou
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  4. Qiang Li
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  5. Peizhi Zhou
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Correspondence to Jianguo Xu.

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Xu, J., You, C., Zhou, L. et al. The cell-cycle kinetics of craniopharyngioma and its clinical significance. J Neurooncol 98, 71–76 (2010). https://doi.org/10.1007/s11060-009-0047-6

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  • DOI: https://doi.org/10.1007/s11060-009-0047-6

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