Abstract
Leptomeningeal (LM) carcinomatosis is an increasing clinical complication in patients with advanced breast cancer (BC). The LM carcinomatosis diagnostic procedures rely mainly on cerebrospinal fluid (CSF) cytology, although both the amount of CSF and the number of malignant cells remain limiting factors. Therefore, efforts should be made to design new highly sensitive diagnostic tools to detect malignant cells in CSF of BC patients with LM carcinomatosis. In this study, the human Mammaglobin (hMAM) mRNA amplification by RT-PCR was employed to detect metastatic cells in CSF and thus, to diagnose LM carcinomatosis in a BC patient. Our data demonstrate that hMAM transcripts are expressed in the CSF of a BC patient with LM carcinomatosis, hence making RT-PCR for hMAM a potentially suitable test to identify occult BC cells in the brain.
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References
Gavrilovic IT, Posner JB (2005) Brain metastases: epidemiology and pathophysiology. J Neurooncol 75:5–14. doi:10.1007/s11060-004-8093-6
Taillibert S, Laigle-Donadey F, Chodkiewicz C et al (2005) Leptomeningeal metastases from solid malignancy: a review. J Neurooncol 75:85–99. doi:10.1007/s11060-004-8101-x
Kesari S, Batchelor TT (2003) Leptomeningeal metastases. Neurol Clin 21:25–66. doi:10.1016/S0733-8619(02)00032-4
Tsukada Y, Fouad A, Pickren JW, Lane WW (1983) Central nervous system metastasis from breast carcinoma. Autopsy study. Cancer 52:2349–2354. doi:10.1002/1097-0142(19831215)52:12<2349::AID-CNCR2820521231>3.0.CO;2-B
DeAngelis L, Rogers L, Foley KM (2000) Leptomeningeal metastasis. In: Harris JR (ed) Disease of the breast. Lippincott Williams & Wilkins, Philadelphia, pp 867–874
Chang EL, Lo S (2003) Diagnosis and management of central nervous system metastases from breast cancer. Oncologist 8:398–410. doi:10.1634/theoncologist.8-5-398
Chiara S, Straathof M, de Bruin HG et al (1999) The diagnostic accuracy of magnetic resonance imaging and cerebrospinal fluid cytology in leptomeningeal metastasis. J Neurol 246:810–814. doi:10.1007/s004150050459
Jeyapalan SA, Batchelor TT (2000) Diagnostic evaluation of neurologic metastases. Cancer Invest 18:381–394. doi:10.3109/07357900009012181
Swinkels DW, de Kok JB, Hanselaar A et al (2000) Early detection of leptomeningeal metastasis by PCR examination of tumor-derived K-ras DNA in cerebrospinal fluid. Clin Chem 46:132–133
Gleissner B, Siehl J, Korfel A et al (2002) CSF evaluation in primary CNS lymphoma patients by PCR of the CDR III IgH genes. Neurology 58:390–396
Rhodes CH, Glantz MJ, Glantz L et al (1996) A comparison of polymerase chain reaction examination of cerebrospinal fluid and conventional cytology in the diagnosis of lymphomatous meningitis. Cancer 77:543–548. doi:10.1002/(SICI)1097-0142(19960201)77:3<543::AID-CNCR17>3.0.CO;2-4
Waston MA, Fleming TP (1996) Mammaglobin, a mammary-specific member of the uteroglobin gene family, is overexpressed in human breast cancer. Cancer Res 56:860–865
Zehentner BK, Carter D (2004) Mammaglobin: a candidate diagnostic marker for breast cancer. Clin Biochem 37:249–257. doi:10.1016/j.clinbiochem.2003.11.005
Roncella S, Ferro P, Bacigalupo B et al (2005) Human mammaglobin mRNA is a reliable molecular marker for detecting occult breast cancer cells in peripheral blood. J Exp Clin Cancer Res 24:265–2671
Raj GV, Moreno JG, Gomella LG (1998) Utilization of polymerase chain reaction technology in the detection of solid tumors. Cancer 15:1419–1442. doi:10.1002/(SICI)1097-0142(19980415)82:8<1419::AID-CNCR1>3.0.CO;2-4
Pelkey TJ, Frierson HF Jr, Bruns DE (1996) Molecular and immunological detection of circulating tumor cells and micrometastases from solid tumors. Clin Chem 42:1369–1381
Mikhitarian K, Martin RH, Ruppel MB et al (2008) Detection of mammaglobin mRNA in peripheral blood is associated with high grade breast cancer: interim results of a prospective cohort study. BMC Cancer 8:55. http://www.biomedcentral.com/1471-2407/8/55. doi:10.1186/1471-2407-8-55
Roncella S, Ferro P, Bacigalupo B et al (2008) Assessment of RT-PCR detection of human mammaglobin for the diagnosis of breast cancer derived pleural effusions. Diagn Mol Pathol 17:28–33
Chamberlain MC, Kormanik PR (1997) Carcinomatous meningitis secondary to breast cancer: predictors of response to combined modality therapy. J Neurooncol 35:55–64. doi:10.1023/A:1005803918194
Kosmas C, Tsavaris NB, Tsakonas G et al (2005) Cerebrospinal fluid tumor marker levels in predicting response to treatment and survival of carcinomatous meningitis in patients with advanced breast cancer. Med Sci Monit 11:398–401
Hao X, Sun B, Hu L et al (2004) Differential gene and protein expression in primary breast malignancies and their lymph node metastases as revealed by combined cDNA microarray and tissue microarray analysis. Cancer 100:1110–1122. doi:10.1002/cncr.20095
Acknowledgements
This work was supported by grants from the Associazione Italiana Contro le Leucemie/Linfoma e Mieloma (AIL, Sezione Francesca Lanzone), Comitato Assistenza Malati Ing. Perioli, La Spezia; and Comitato Assistenza Malati e Lotta Contro i Tumori, Sarzana (La Spezia); Italy.
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Dono, M., Ferro, P., Benedetti, L. et al. Molecular detection of human mammaglobin in cerebrospinal fluid from breast cancer patient with leptomeningeal carcinomatosis. J Neurooncol 91, 295–298 (2009). https://doi.org/10.1007/s11060-008-9711-5
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DOI: https://doi.org/10.1007/s11060-008-9711-5