Abstract
Objective The metabolism of malignant glioma was studied in 13 patients. The main objective was to perform a study of the metabolic pattern of glucose, lactate, pyruvate, glutamate and glycerol in tumour tissue during base-line conditions and to detect any changes in the metabolism during radiotherapy. Method During a stereotactic biopsy, two microdialysis catheters were implanted: one in tumour and one in peri-tumoural tissue. Fasting samples were analysed daily, before and during 5 days of radiotherapy given with 2 Gy fractions. Results Base-line levels of glucose and pyruvate were significantly lower in tumour compared to peri-tumoural tissue (P = 0.04 and 0.023, respectively). The lactate/pyruvate ratio was significantly higher in tumour tissue (P = 0.022). In general, the levels of lactate, glutamate and glycerol were higher in tumour tissue, although not statistically significant. Further, we could not detect any significant changes during the 5 days of radiotherapy in any of the metabolites analysed. Conclusion Radiotherapy up to 10 Gy given in five fractions does not influence the glucose metabolism nor does it induce any acute cytotoxic effect detected with glutamate or glycerol in malignant glioma, as assessed by microdialysis. The study confirms the glycolytic properties of glucose metabolism in malignant glioma.
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References
Behrens PF, Langemann H, Strohschein R et al (2000) Extracellular glutamate and other metabolites in and around RG2 rat glioma: an intracerebral microdialysis study. J Neurooncol 47:11–22
Bergenheim AT, Capala J, Roslin M et al (2005) Distribution of BPA and metabolic assessment in glioblastoma patients during BNCT treatment: a microdialysis study. J Neurooncol 71:287–293
Butler AB, Brooks WH, Netsky MG (1982) Classification and biology of brain tumours. I.. WB Saunders, Philadelphia, PA
Cho Y, Bannai S (1990) Uptake of glutamate and cysteine in C-6 glioma cells and in cultured astrocytes. J Neurochem 55:2091–2097
Delorme S, Weber MA (2006) Applications of MRS in the evaluation of focal malignant brain lesions. Cancer Imaging 6:95–99
Hillered L, Valtysson J, Enblad P et al (1998) Interstitial glycerol as a marker for membrane phospholipid degradation in the acutely injured human brain. J Neurol Neurosurg Psychiatr 64:486–491
Klegeris A, Walker DG, McGeer PL (1997) Regulation of glutamate in cultures of human monocytic THP-1 and astrocytoma U-373 MG cells. J Neuroimmunol 78:152–161
Lichtor T, Dohrmann GJ (1986) Respiratory patterns in human brain tumors. Neurosurgery 19:896–899
Mangiardi JR, Yodice P (1990) Metabolism of the malignant astrocytoma. Neurosurgery 26:1–19
Maruyama I, Sadato N, Waki A et al (1999) Hyperacute changes in glucose metabolism of brain tumors after stereotactic radiosurgery: a PET study. J Nucl Med 40:1085–1090
Rhodes CG, Wise RJ, Gibbs JM et al (1983) In vivo disturbance of the oxidative metabolism of glucose in human cerebral gliomas. Ann Neurol 14:614–626
Roslin M, Henriksson R, Bergstrom P et al (2003) Baseline levels of glucose metabolites, glutamate and glycerol in malignant glioma assessed by stereotactic microdialysis. J Neurooncol 61:151–160
Seyfried TN, Mukherjee P (2005) Targeting energy metabolism in brain cancer: review and hypothesis. Nutr Metab (Lond) 2:30
Sokoloff L, Reivich M, Kennedy C et al (1977) The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28:897–916
Stupp R, Dietrich PY, Ostermann Kraljevic S et al (2002) Promising survival for patients with newly diagnosed glioblastoma multiforme treated with concomitant radiation plus temozolomide followed by adjuvant temozolomide. J Clin Oncol 20:1375–1382
Wibom C, Pettersson F, Sjostrom M et al (2006) Protein expression in experimental malignant glioma varies over time and is altered by radiotherapy treatment. Br J Cancer 95:766
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The technical assistance of Kristin Nyman and Sonja Edvinsson is acknowledged. The authors are grateful for financial support by Lions Cancer Foundation and Umeå University.
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Tabatabaei, P., Bergström, P., Henriksson, R. et al. Glucose metabolites, glutamate and glycerol in malignant glioma tumours during radiotherapy. J Neurooncol 90, 35–39 (2008). https://doi.org/10.1007/s11060-008-9625-2
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DOI: https://doi.org/10.1007/s11060-008-9625-2