Skip to main content

Advertisement

SpringerLink
Log in

Discovery of serum biomarkers in astrocytoma by SELDI–TOF MS and proteinchip technology

  • Clinical Study - Patient Studies
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

Objective

To discover serum biomarkers in astrocytoma patients for early detection of glioma and evaluation of prognosis.

Methods

A total of 140 serum samples were analyzed using the weak cation-exchange (WCX) chips. Among those, 73 were sera from astrocytoma patients, 56 from normal controls, and 11 from other brain tumors. For building a diagnosis model, the samples were randomly split into a training set and a test set with similar distribution of case and control samples. Proteomics profiles were obtained using a PBSII instrument and the data were analyzed with the accompanying software.

Results

Seven serum biomarkers were significantly deregulated in astrocytoma group comparing to the normal control group. Among them, four were up-regulated and three were down-regulated. A decision tree classification method were developed using these seven markers. A sensitivity of 84.6% and a selectivity of 84.6% were achieved to discriminate astrocytoma from normal controls. In addition, a correlation of these markers with the astrocytoma malignancy was observed.

Conclusions

Proteomics approaches such as SELDI–TOF mass spectrometry could greatly facilitate the discovery of serum biomarkers in astrocytoma. The discovered biomarkers might show great potential for early detection of astrocytoma and evaluation of prognosis for those clinical suspect astrocytoma patients. However, this need warrant further study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

Abbreviations

CV:

Coefficient of variance

ExPASy:

Expert protein analysis system

M/Z:

Mass/charge

PBS:

Proteinchip® biomarker system

SELDI–TOF MS:

Surface-enhanced laser desorption/ionization time of flight mass spectrometry

SIB:

Swiss institute of bioinformatics

WCX:

Weak cation-exchange

References

  1. Merchant M, Weinberger SR (2000) Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis 21:1164–1177

    Article  PubMed  CAS  Google Scholar 

  2. Wiesner A (2004) Detection of tumor markers with proteinchip technology. Curr Pharm Biotechnol 5(1):45–67

    Article  PubMed  CAS  Google Scholar 

  3. Bast RC Jr (2003) Status of tumor markers in ovarian cancer screening. J Clin Oncol 21(10 Suppl):200–205

    Article  Google Scholar 

  4. Cazares LH, Adam BL, Ward MD, Nasim S, Schellhammer PF, Semmes OJ, Wright GLJ (2002) Normal, benign, preneoplastic, and malignant prostate cells have distinct protein expression profiles resolved by Surface Enhanced Laser Desorption/Ionization mass spectrometry. Clin Cancer Res 8(8):2541–2552

    PubMed  CAS  Google Scholar 

  5. Mannes AJ, Martin BM, Yang HY, Keller JM, Lewin S, Gaiser RR, Iadarola MJ (2003) Cystatin C as a cerebrospinal fluid biomarker for pain in humans. Pain 102(3):251–256

    Article  PubMed  CAS  Google Scholar 

  6. Li J, Zhang Z, Rosenzweig J, Wang YY, Chan DW (2002) Proteomics and bioinformatics approaches for identification of serum biomarkers to detect breast cancer. Clin Chem 48:1296–1304

    PubMed  CAS  Google Scholar 

  7. Srinivas PR, Verma M, Zhao Y, Srivastava S, Clark RA, Tockman MS (2002) Proteomics for cancer biomarker discovery. Clin Chem 48(8):1160–1169

    PubMed  CAS  Google Scholar 

  8. Hutchens TW, Yip TT (1993) New desorption stratefies for the mass spectrometric analysis of macromolecules. Rapid Commun Mass Spectrum 7:576–580

    Article  CAS  Google Scholar 

  9. Adam BL, Qu Y, Davis JW, Ward MD, Clements MA, Cazares LH, Semmes OJ, Schellhammer PF, Yasui Y, Feng Z, Wright GL Jr (2002) Serum protein fingerprinting coupled with a pattern-matching algorithm distinguishes prostate cancer from benigh prostate gyperplasia and healthy men. Cancer Res 62:3609–3614

    PubMed  CAS  Google Scholar 

  10. Petricoin EF 3rd, Ornstein DK, Paweletz CP, Ardekani A, Hackett PS, Hitt BA, Velassco A, Trucco C, Wiegand L, Wood K, Simone CB, Levine PJ, Linehan WM, Emmert-Buck MR, Steinberg SM, Kohn EC, Liotta LA (2002) Serum proteomic patterns for detection of prostate cancer. J Nat Cancer Inst 94:1576–1578

    PubMed  CAS  Google Scholar 

  11. Xiao Z, Adam BL, Cazares LH, Clements MA, Davis JW, Schellhammer PF, Dalmasso EA, Wright GL Jr (2001) Quantitation of serum prostate-specific membrane antigen by a novel protein biochip immunoassay discriminates benign from malignant prostate disease. Cancer Res 61:6029–6033

    PubMed  CAS  Google Scholar 

  12. Vlahou A, Schellhammer PF, Mendrinos S, Patel K, Kondylis FI, Gong L, Nasim S, Wright GL Jr (2001) Development of a novel proteomic approach for the detection of transitional cell carcinoma of the bladder in urine. Am J Pathol 158:1491–1502

    PubMed  CAS  Google Scholar 

  13. Bandera CA, Ye B, Mok SC (2003) New technologies for the identification of markers for early detection of ovarian cancer. Curr Opin Obstet Gynecol 15:51–55

    Article  PubMed  Google Scholar 

  14. Petricoin EF, Ardekani AM, Hitt BA, Levine PJ, Fusaro VA, Steinberg SM, Mills GB, Simone C, Fishman DA, Kohn EC, Liotta LA (2002) Use of proteomic patterns in serum to identify ovarian cancer. Lancet 359:572–577

    Article  PubMed  CAS  Google Scholar 

  15. Coombes KR, Fritsche HA Jr, Clarke C, Chen JN, Baggerly KA, Morris JS, Xiao LC, Hung MC, Kuerer HM (2003) Quality control and peak finding for proteomics data collected from nipple aspirate fluid by surface-enhanced laser desorption and ionization. Clin Chem 49:1615–1623

    Article  PubMed  CAS  Google Scholar 

  16. Xiao XY, Tang Y, Wei XP, He DC (2003) A preliminary analysis of non-small cell lung cancer biomarkers in serum. Biomed Environ Sci 16:140–148

    PubMed  Google Scholar 

  17. Rosty C, Christa L, Kuzdzal S, Baldwin WM, Zahurak ML, Carnot F, Chan DW, Canto M, Lillemoe KD, Cameron JL, Yeo CJ, Hruban RH, Goggins M (2002) Identification of Hepatocarcinoma-Intestine-Pancreas/Pancreatitis—associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. Cancer Res 62:1868–1875

    PubMed  CAS  Google Scholar 

  18. Pauleit D, Floeth F, Hamacher K, Riemenschneider MJ, Reifenberger G, Muller HW, Zilles K, Coenen HH, Langen KJ (2005) O-(2-[18F]fluoroethyl)-l-tyrosine PET combined with MRI improves the diagnostic assessment of cerebral gliomas. Brain 128:678–687

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Huashan Hospital, Fudan University, No. 12 WuLuMuQi Zhong Road, Shanghai, 200040, P.R. China

    Haishi Zhang, Gang Wu & Fengping Huang

  2. Shanghai Cancer Institute, Shanghai Jiaotong University, No. 2200 XieTu Road, Shanghai, P.R. China

    Hong Tu

Authors
  1. Haishi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gang Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hong Tu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fengping Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fengping Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, H., Wu, G., Tu, H. et al. Discovery of serum biomarkers in astrocytoma by SELDI–TOF MS and proteinchip technology. J Neurooncol 84, 315–323 (2007). https://doi.org/10.1007/s11060-007-9376-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11060-007-9376-5

Keywords

Search

Navigation