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LRRC4 controls in vitro invasion of glioblastoma cells through inhibiting RPTP-zeta expression

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Abstract

LRRC4 (leucine rich repeat containing 4), a novel member of LRP (Leucine-rich repeat protein) superfamily, contains a conserved leucine-rich repeat (LRR) cassette and an immunoglobulin-like (IgC2) domain in its extracellular region. In the present study, we demonstrated that the N and C terminal LRR (LRRNT and LRRCT) are requisite for membrane and cytoplasm location of LRRC4 in Cos7 cells. We also suggested that RPTP-zeta (receptor-type protein tyrosine phosphatase) receptor is relevant to the invasion ability of gliomas cells, and its expression is inhibited by the reexpression of LRRC4. Our observations indicated that LRRC4 may be a negative regulator of the RPTP-zeta receptor, and contribute to suppressing the invasion ability of gliomas cells.

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Abbreviations

LRRC4:

Leucine rich repeat containing 4

LRR:

Leucine rich repeat

LRP:

Leucine-rich repeat proteins

LRRNT:

N-terminal LRR

LRRCT:

C-terminal LRR

OMgp:

The oligodendrocyte myelin glycoprotein

CRM1:

(Chromosome region maintenance)/exportin1

NES:

Nuclear export signal

LMB:

Leptomycin B

SLDT:

Scrape-loading and dye transfer

GJIC:

Gap junctional intercellular communication

RPTP:

Receptor-type protein tyrosine phosphatase

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Acknowledgements

The authors are grateful to Dr. Jianbo Yang (The university of Minnesota ) for reviewing this paper, and this work was supported by grants from the National Science Foundation of China (No. 30500192, 30330560, 30500444, 30500295) and Hunan Province Natural Sciences Foundations of China (No. 05JJ40059).

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Authors and Affiliations

  1. Cancer Research Institute, Central South University, 110# Xiang-Ya Road, Changsha , 410078, Hunan, People’s Republic of China

    Minghua Wu, Kai Gan, Chen Huang, Yunlian Tang, Qiong Chen, Ke Tang, Xiaoling Li & Guiyuan Li

  2. The Third Affiliated Hospital of Xiang Ya School of Medicine, Central South University, Changsha , 410078, Hunan, People’s Republic of China

    Shourong Shen

Authors
  1. Minghua Wu
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  2. Kai Gan
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  3. Chen Huang
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  4. Yunlian Tang
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  5. Qiong Chen
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  6. Ke Tang
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  7. Xiaoling Li
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  8. Shourong Shen
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  9. Guiyuan Li
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Corresponding author

Correspondence to Guiyuan Li.

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Minghua Wu, Kai Gan, Chen Huang contributed equally to this work.

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Wu, M., Gan, K., Huang, C. et al. LRRC4 controls in vitro invasion of glioblastoma cells through inhibiting RPTP-zeta expression. J Neurooncol 80, 133–142 (2006). https://doi.org/10.1007/s11060-006-9173-6

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  • DOI: https://doi.org/10.1007/s11060-006-9173-6

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