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Dendritic Cells Pulsed with Total Tumor RNA for Activation NK-like T Cells Against Glioblastoma Multiforme

  • Laboratory Investigation
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Summary

Dendritic cells (DCs) are potent antigen presenting cells and play critical role in T cell-mediated immunity. DCs have been shown to induce strong anti-tumor responses both in vitro and in vivo. Their efficacies in tumor therapy are being investigated in clinical trials. Previous evidence has shown that these DCs enhance the cytotoxicity of NK cells. We generated NK-like T cells (CD3+CD56+), a novel type of effector cells differentiated from normal lymphocyte, which is now being used for adoptive immunotherapy in clinical trials. This study aimed to elucidate the effects of NK-like T cells after co-culturing with DCs against tumor cells. The result revealed that tumor-derived RNA-pulsed DCs can enhance the immune responses of NK-like T cells against glioblastoma multiforme cell line but these effector cells did not appear to have the cytotoxic effect against normal cells (human umbilical vein endothelial cells (HUVEC) and fibroblasts) in vitro. This study may be beneficial for the development of new immunologic effector cells for using in adoptive immunotherapy for glioblastoma multiforme in the future.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

    Prachya Vichchatorn & Sumalee Tangpradabkul

  2. Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand

    Adisak Wongkajornsilp

  3. Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Bangkok, Thailand

    Sawang Petvises

  4. Department of Pediatrics, and Bone Marrow Transplant Program, Ramathibodi Hospital, Faculty of Medicine, Mahidol University, Bangkok, Rama VI Rd, 10400, Thailand

    Samart Pakakasama & Suradej Hongeng

  5. Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand

    Suradej Hongeng

Authors
  1. Prachya Vichchatorn
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  2. Adisak Wongkajornsilp
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  3. Sawang Petvises
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  4. Sumalee Tangpradabkul
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  5. Samart Pakakasama
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  6. Suradej Hongeng
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Correspondence to Suradej Hongeng.

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Vichchatorn, P., Wongkajornsilp, A., Petvises, S. et al. Dendritic Cells Pulsed with Total Tumor RNA for Activation NK-like T Cells Against Glioblastoma Multiforme. J Neurooncol 75, 111–118 (2005). https://doi.org/10.1007/s11060-005-2317-2

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  • DOI: https://doi.org/10.1007/s11060-005-2317-2

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