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Quantification of thrombospondin-1 secretion and expression of αvβ3 and α3β1 integrins and syndecan-1 as cell-surface receptors for thrombospondin-1 in malignant glioma cells

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Abstract

Malignant glioma cells secrete thrombospondin-1 (TSP-1) which participates in the motility of glioma cells, and binds to cell surface αvβ3 and α3β1 integrins, and syndecan-1. This study evaluated the amount of TSP-1 secretion from malignant glioma cells, and the expression of αvβ3 and α3β1 integrins, and syndecan-1. The amounts of TSP-1 in the supernatants from 10 malignant glioma cell lines and eight non-glioma malignant tumor cell lines were measured by enzyme-linked immunosorbent assay. Expression of αvβ3 and α3β1 integrins, and syndecan-1 were examined by flow cytometry. The amounts of TSP-1 secreted by malignant glioma cells were 43 to 2431 ng/1 × 106 cells/24 h (mean ± SD=626 ± 792). Seven of 10 glioma cell lines secreted more than 100 ng of TSP-1 and three of these cell lines secreted more than 1 μg. Seven of eight non-glioma cell lines secreted less than 100 0ng of TSP-1. All glioma cell lines expressed α3β1 integrin and syndecan-1, and seven of 10 glioma cell lines expressed αvβ3 integrin. Treatment of the glioma cell lines with TGF-β2 did not change the expression of αvβ3 integrin. These results suggest that malignant glioma cells secrete high levels of TSP-1, which may be important in the migration of glioma cells via interactions with αvβ3 and α3β1 integrins, and syndecan-1.

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Authors and Affiliations

  1. Department of Neurosurgery, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Tamaho-machi, Nakakoma-gun, Yamanashi, 409-3898, Japan

    Hirofumi Naganuma M.D., Ph.D., Eiji Satoh, Takayuki Asahara, Kenichi Amagasaki, Arata Watanabe, Hiroki Satoh, Katsuhiro Kuroda, Lei Zhang & Hideaki Nukui

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  1. Hirofumi Naganuma M.D., Ph.D.
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  2. Eiji Satoh
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  3. Takayuki Asahara
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  4. Kenichi Amagasaki
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  5. Arata Watanabe
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  6. Hiroki Satoh
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  7. Katsuhiro Kuroda
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  8. Lei Zhang
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  9. Hideaki Nukui
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Correspondence to Hirofumi Naganuma M.D., Ph.D..

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Naganuma, H., Satoh, E., Asahara, T. et al. Quantification of thrombospondin-1 secretion and expression of αvβ3 and α3β1 integrins and syndecan-1 as cell-surface receptors for thrombospondin-1 in malignant glioma cells. J Neurooncol 70, 309–317 (2004). https://doi.org/10.1007/s11060-004-9167-1

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  • DOI: https://doi.org/10.1007/s11060-004-9167-1

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