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Immunohistochemical study of central neurocytoma, subependymoma, and subependymal giant cell astrocytoma

  • Laboratory Investigation
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Summary

For investigation of histogenesis of central neurocytomas (CNs), subependymoma (SEs), subependymal giant cell astrocytomas (SEGAs), we studied expression of various neuronal and glial biomarkers by immunohistochemical (IHC) study and reverse transcriptase-polymerase chain reaction (RT-PCR). The materials for IHC␣were paraffin section of seven CNs, three SEs, and eight SEGAs and those for RT-PCR were frozen tissues of seven CNs, three SEs, and five SEGAs. Control group was five ependymomas (EPs) and four pilocytic astrocytomas (PAs). The neuronal biomarkers included nestin, chromogranin A (chrA), synaptophysin (SNP), neuronal cell adhesion molecule (NCAM), neuron specific enolase (NSE), neuronal nuclear antigen (NeuN), neurofilament (NF) and the glial marker was GFAP. CNs expressed all neuronal markers except NF (0%), SNP (100%), NCAM (100%), NSE (100%), NeuN (100%), nestin (29%) and chrA (43%), but GFAP expression was found only in one case (14%). SEGA coexpressed several neuronal markers and a glial marker; NeuN (100%), NSE (88%), NCAM (63%), nestin (100%), SNP (weakly and focally, 100%), and GFAP (100%), however, other neuronal markers including chrA, SNP and NF were all negative. SE expressed nonspecific neuronal markers (NCAM (100%) and NSE (100%)) which showed weak intensity and a GFAP (100%), but not nestin. Among control cases of EPs and PAs, no one case expressed neuronal markers except nonspecific neuronal marker of NCAM, but robustly expressed GFAP. RT-PCR product of nestin was expressed in 29% of CNs (2/7cases), 60% of SEGAs (3/5 cases), 100% of SEs (3/3 cases), 80% of EPs (4/5 cases), and 25% of PAs (1/4 cases). Conclusively, coexpression of neuronal and glial markers and expression of nestin in CNs, SEGAs and SEs suggested the origin of these tumor cells might be the stem cells being able to differentiate into both neuronal and glial phenotypes. But CNs might be originated from rather neuronally committed stem cells and SEs from rather glially committed stem cells.

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Authors and Affiliations

  1. Neuro-Oncology Clinic, Center for Specific Organ Center, National Cancer, Seoul, Korea

    Heon You

  2. Department of Neuro-surgery, Department of Pathology, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, 28 Yongon-Dong, Chongno-Gu, Seoul, 110-744, Seoul, Republic of Korea

    Young Im Kim, Soo Young Im, Sun Ha Paek, Sung-Hye Park, Dong Gyu Kim & Hee-Won Jung

  3. Department of Anatomy, School of Medicine, Ajou University, Suwon, Korea

    Haeyoung Suh-Kim

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  1. Heon You
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  2. Young Im Kim
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  3. Soo Young Im
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  4. Haeyoung Suh-Kim
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  6. Sung-Hye Park
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  8. Hee-Won Jung
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Correspondence to Sun Ha Paek.

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You, H., Kim, Y.I., Im, S.Y. et al. Immunohistochemical study of central neurocytoma, subependymoma, and subependymal giant cell astrocytoma. J Neurooncol 74, 1–8 (2005). https://doi.org/10.1007/s11060-004-2354-2

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