Preconditioning using three sessions of moderate hypobaric hypoxia, i.e., hypoxic preconditioning (HP), increased the tolerance of susceptible brain neurons to severe hypoxia and other harmful factors. The study addressed changes in the expression of transcription factors NF-κB (nuclear factor kappa B) and CREB (cAMP response element binding protein) in the hippocampus of rats preconditioned with moderate hypoxia. Immunocytochemical methods demonstrated that HP increased immunoreactivity for NF-κB and phosphorylated CREB (pCREB) in hippocampal fields CA1–CA4 and the dentate gyrus and promoted increases in the expression of these transcription factors in the hippocampus of preconditioned rats 3–24 h after severe hypobaric hypoxia. These data provide evidence that NF-κB and CREB are involved in the mechanisms forming HP-induced tolerance of the brain.
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References
M. O. Samoilov, The Bran and Adaptation. Molecular-Genetic Mechanisms [in Russian], Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg (1999).
S. A. Stroev and M. O. Samoilov, Endogenous Antioxidants and the Hypoxic Tolerance of the Brain [in Russian], Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg (2006).
N. Blondeau, C. Widmann, M. Lazdunski, and C. Heurteaux, “Activation of the nuclear factor-kappaB is a key event in brain tolerance,” J. Neurosci., 21, 4668–4677 (2001).
G. I. Botchkina, E. Geimonen, M. L. Bilof, et al., “Loss of NFkappaB activity during cerebral ischemia and TNF cytotoxicity,” J. Mol. Med., 5, 372–381 (1999).
N. T. Bui, A. Livolsi, J. F. Peyron, and J. H. Prehn, “Activation of nuclear factor kappaB and Bcl-x survival gene expression by nerve growth factor requires tyrosine phosphorylation of IkappaBalpha,” J. Cell Biol., 152, 753–764 (2001).
C. C. Chiueh, T. Andoh, and P. B. Chock, “Induction of thioredoxin and mitochondrial survival proteins mediates preconditioninginduced cardioprotection and neuroprotection,” Ann. N.Y. Acad. Sci., 1042, 403–418 (2005).
T. Hara, J. Hamada, S. Yano, et al., “CREB is required for acquisition of ischemic tolerance in gerbil hippocampal CA1 region,” J. Neurochem., 86, 805–814 (2003).
S. Kida, S. A. Josselyn, S. P. de Ortiz, et al., “CREB required for the stability of new and reactivated fear memories, “Nat. Neurosci., 5, 348–355 (2002).
B. E. Lonze and D. D. Ginty, “Function and regulation of CREB family transcription factors in the nervous system,” Neuron, 35, 605–623 (2002).
M. P. Mattson, “NF-kappaB in the survival and plasticity of neurons,” Neurochem. Res., 30, 883–893 (2005).
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, Sydney, Orlando, San Diego, New York, Austin, London, Montreal, Tokyo, Toronto (1986).
E. Rybnikova, T. Gluschenko, E. Tjulkova, et al., “Preconditioning induces prolonged expression of transcription factors pCREB and NF-kappaB in the neocortex of rats before and following severe hypoxia,” J. Neurochem., 106, 1450–1458 (2008).
E. Rybnikova, N. Sitnik, T. Gluschenko, et al., “The preconditioning modified neuronal expression of apoptosis-related proteins of Bcl-2 superfamily following severe hypobaric hypoxia in rats,” Brain Res., 1089, 195–202 (2006).
E. Rybnikova, L. Vataeva, E. Tyulkova, et al., “Preconditioning prevents impairment of passive avoidance learning and suppression of brain NGFI-A expression induced by severe hypoxia,” Behav. Brain Res., 160, 107–114 (2005).
Z. Yu, D. Zhou, A. J. Bruce-Keller, et al., “Lack of the p50 subunit of nuclear factor-kappaB increases the vulnerability of hippocampal neurons to excitotoxic injury,” J. Neurosci., 19, 8856–8865 (1999).
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Translated from Morfologiya, Vol. 136, No. 6, pp. 38–42, November–December, 2009.
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Churilova, A.V., Rybnikova, E.A., Glushchenko, T.S. et al. Effects of Moderate Hypobaric Hypoxic Preconditioning on the Expression of the Transcription Factors pCREB and NF-κB in the Rat Hippocampus Before and After Severe Hypoxia. Neurosci Behav Physi 40, 852–857 (2010). https://doi.org/10.1007/s11055-010-9340-2
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DOI: https://doi.org/10.1007/s11055-010-9340-2