Abstract
Functionalized graphene oxide (fGO) was synthesized and subsequently used for synthesis of nanocomposite with polypyrrole (PPy) and chitosan (CS) to give fGO–PPy–CS nanocomposites. Screen-printed carbon electrodes were then modified with these nanocomposites to construct an unprecedented hydrogen peroxide (H2O2) sensor. Cyclic voltammetry (CV) and amperometric response demonstrated that the composite materials hold potential for electrocataytic reduction towards H2O2. Under optimal experimental conditions, the amperometric response of the fGO–PPy–CS sensor was linearly proportional to H2O2 in the range of 2.5–200 μM with a detection limit of 1.95 μM (S/N = 3). The present study provides new opportunities for fGO–PPy–CS-modified electrodes to probe the improved non-enzymatic detection of H2O2. It further broadens the applications of graphene-based conducting composites in the field of sensors and biosensors for diverse applications.
The present study provides new opportunities for fGO–PPy–CS-modified electrodes to probe the improved non-enzymatic detection of H2O2.
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References
Amanulla B, Palanisamy S, Chen SM, Velusamy V, Chiu TW, Chen TW, Ramaraj SK (2017) A non-enzymatic amperometric hydrogen peroxide sensor based on iron nanoparticles decorated reduced graphene oxide nanocomposite. J Colloid Interface Sci 487:370–377. https://doi.org/10.1016/j.jcis.2016.10.050
Anandhavelu S, Thambidurai S (2013) Single step synthesis of chitin/chitosan-based graphene oxide–ZnO hybrid composites for better electrical conductivity and optical properties. Electrochim Acta 90:194–202. https://doi.org/10.1016/j.electacta.2012.12.024
Bohrer FI, Colesniuc CN, Park J, Schuller IK, Kummel AC, Trogler WC (2008) Selective detection of vapor phase hydrogen peroxide with phthalocyanine chemiresistors. J Am Chem Soc 130(12):3712–3713. https://doi.org/10.1021/ja710324f
Chang MCY, Pralle A, Isacoff EY, Chang CJ (2004) A selective, cell-permeable optical probe for hydrogen peroxide in living cells. J Am Chem Soc 126(47):15392–15393. https://doi.org/10.1021/ja0441716
Chen KJ, Chandrasekara Pillai K, Rick J, Pan CJ, Wang SH, Liu CC, Hwang BJ (2012) Bimetallic PtM (M=Pd, Ir) nanoparticle decorated multi-walled carbon nanotube enzyme-free, mediator-less amperometric sensor for H(2)O(2). Biosens Bioelectron 33(1):120–127. https://doi.org/10.1016/j.bios.2011.12.037
Deng M, Yang X, Silke M, Qiu W, Xu M, Borghs G, Chen H (2011) Electrochemical deposition of polypyrrole/graphene oxide composite on microelectrodes towards tuning the electrochemical properties of neural probes. Sensors Actuators B Chem 158(1):176–184. https://doi.org/10.1016/j.snb.2011.05.062
Diaz-Diestra D, Beltran-Huarac J, Bracho-Rincon DP, Gonzalez-Feliciano JA, Gonzalez CI, Weiner BR, Morell G (2015) Biocompatible ZnS:Mn quantum dots for reactive oxygen generation and detection in aqueous media. J Nanopart Res 17(12):461. https://doi.org/10.1007/s11051-015-3269-x
Dong Y (2016) H2O2 electrochemical sensor based on Scutellaria Barbata extract biosynthesized of gold nanoparticles. Int J Electrochem Sci 11:10919–10927. 10.20964/2016.12.80
Dong S, Xi J, Wu Y, Liu H, Fu C, Liu H, Xiao F (2015) High loading MnO2 nanowires on graphene paper: Facile electrochemical synthesis and use as flexible electrode for tracking hydrogen peroxide secretion in live cells. Anal Chim Acta 853:200–206. https://doi.org/10.1016/j.aca.2014.08.004
Fonner JM, Forciniti L, Nguyen H, Byrne JD, Kou YF, Syeda-Nawaz J, Schmidt CE (2008) Biocompatibility implications of polypyrrole synthesis techniques. Biomed Mater 3(3):034124. https://doi.org/10.1088/1748-6041/3/3/034124
Gao W, Tjiu WW, Wei J, Liu T (2014) Highly sensitive nonenzymatic glucose and H2O2 sensor based on Ni(OH)2/electroreduced graphene oxide--multiwalled carbon nanotube film modified glass carbon electrode. Talanta 120:484–490. https://doi.org/10.1016/j.talanta.2013.12.012
Golsheikh AM, Huang NM, Lim HN, Zakaria R (2014) One-pot sonochemical synthesis of reduced graphene oxide uniformly decorated with ultrafine silver nanoparticles for non-enzymatic detection of H2O2and optical detection of mercury ions. RSC Adv 4(69):36401–36411. https://doi.org/10.1039/c4ra05998k
Guo Y, Yang L, Li W, Wang X, Shang Y, Li B (2016) Carbon dots doped with nitrogen and sulfur and loaded with copper(II) as a “turn-on” fluorescent probe for cystein, glutathione and homocysteine. Microchim Acta 183(4):1409–1416. https://doi.org/10.1007/s00604-016-1779-6
Halliwell B, Clement MV, Long LH (2000) Hydrogen peroxide in the human body. FEBS Lett 486(1):10–13. https://doi.org/10.1016/S0014-5793(00)02197-9
Heli H, Pishahang J (2014) Cobalt oxide nanoparticles anchored to multiwalled carbon nanotubes: synthesis and application for enhanced electrocatalytic reaction and highly sensitive nonenzymatic detection of hydrogen peroxide. Electrochim Acta 123:518–526. https://doi.org/10.1016/j.electacta.2014.01.032
Hussain M, Sun H, Karim S, Nisar A, Khan M, ul Haq A, Iqbal M, Ahmad M (2016) Noble metal nanoparticle-functionalized ZnO nanoflowers for photocatalytic degradation of RhB dye and electrochemical sensing of hydrogen peroxide. J Nanopart Res 18(4):95. https://doi.org/10.1007/s11051-016-3397-y
Kang X, Wang J, Wu H, Aksay IA, Liu J, Lin Y (2009) Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. Biosens Bioelectron 25(4):901–905. https://doi.org/10.1016/j.bios.2009.09.004
Konwer S, Boruah R, Dolui SK (2011) Studies on conducting polypyrrole/graphene oxide composites as supercapacitor electrode. J Electron Mater 40(11):2248–2255. https://doi.org/10.1007/s11664-011-1749-z
Kuila T, Bose S, Mishra AK, Khanra P, Kim NH, Lee JH (2012) Chemical functionalization of graphene and its applications. Prog Mater Sci 57(7):1061–1105. https://doi.org/10.1016/j.pmatsci.2012.03.002
Lee KK, Loh PY, Sow CH, Chin WS (2013) CoOOH nanosheet electrodes: simple fabrication for sensitive electrochemical sensing of hydrogen peroxide and hydrazine. Biosens Bioelectron 39(1):255–260. https://doi.org/10.1016/j.bios.2012.07.061
Li H, Hao W, Hu J, Wu H (2013) A photoelectrochemical sensor based on nickel hydroxyl-oxide modified n-silicon electrode for hydrogen peroxide detection in an alkaline solution. Biosens Bioelectron 47:225–230. https://doi.org/10.1016/j.bios.2013.03.028
Liu Y, Deng R, Wang Z, Liu H (2012) Carboxyl-functionalized graphene oxide-polyaniline composite as a promising supercapacitor material. J Mater Chem 22(27):13619–13624. https://doi.org/10.1039/C2JM32479B
Liu X, Xu X, Zhu H, Yang X (2013a) Synthesis of graphene nanosheets with incorporated silver nanoparticles for enzymeless hydrogen peroxide detection. Anal Methods 5(9):2298. https://doi.org/10.1039/c3ay26458k
Liu M, Liu R, Chen W (2013b) Graphene wrapped Cu2O nanocubes: non-enzymatic electrochemical sensors for the detection of glucose and hydrogen peroxide with enhanced stability. Biosens Bioelectron 45:206–212. https://doi.org/10.1016/j.bios.2013.02.010
Loryuenyong V, Totepvimarn K, Eimburanapravat P, Boonchompoo W, Buasri A (2013) Preparation and characterization of reduced graphene oxide sheets via water-based exfoliation and reduction methods. Adv Mater Sci Eng 2013:1–5. https://doi.org/10.1155/2013/923403
Miao Y-E, He S, Zhong Y, Yang Z, Tjiu WW, Liu T (2013) A novel hydrogen peroxide sensor based on Ag/SnO2 composite nanotubes by electrospinning. Electrochim Acta 99:117–123. https://doi.org/10.1016/j.electacta.2013.03.063
Mollarasouli F, Asadpour-Zeynali K, Campuzano S, Yáñez-Sedeño P, Pingarrón JM (2017) Non-enzymatic hydrogen peroxide sensor based on graphene quantum dots-chitosan/methylene blue hybrid nanostructures. Electrochim Acta 246:303–314. https://doi.org/10.1016/j.electacta.2017.06.003
Morales-Narvaez E, Baptista-Pires L, Zamora-Galvez A, Merkoci A (2016) Graphene-based biosensors: going simple. Adv Mater. https://doi.org/10.1002/adma.201604905
Morales-Narvaez E, Baptista-Pires L, Zamora-Galvez A, Merkoci A (2017) Graphene-based siosensors: going simple. Adv Mater 29(7):1604905. https://doi.org/10.1002/adma.201604905
Palanisamy S, Chen S-M, Sarawathi R (2012) A novel nonenzymatic hydrogen peroxide sensor based on reduced graphene oxide/ZnO composite modified electrode. Sensors Actuators B Chem 166-167:372–377. https://doi.org/10.1016/j.snb.2012.02.075
Richardson-Burns SM, Hendricks JL, Martin DC (2007) Electrochemical polymerization of conducting polymers in living neural tissue. J Neural Eng 4(2):L6–l13. https://doi.org/10.1088/1741-2560/4/2/l02
Shan C, Yang H, Han D, Zhang Q, Ivaska A, Niu L (2010) Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing. Biosens Bioelectron 25(5):1070–1074. https://doi.org/10.1016/j.bios.2009.09.024
Song XC, Wang X, Zheng YF, Ma R, Yin HY (2011) A hydrogen peroxide electrochemical sensor based on Ag nanoparticles grown on ITO substrate. J Nanopart Res 13(10):5449–5455. https://doi.org/10.1007/s11051-011-0532-7
Su J, Gao F, Gu Z, Pien M, Sun H (2013) A novel 3-D fabrication of platinum nanoparticles decorated micro carbon pillars electrode for high sensitivity detection of hydrogen peroxide. Sensors Actuators B Chem 181:57–64. https://doi.org/10.1016/j.snb.2013.02.023
Sun A, Sheng Q, Zheng J (2012) A hydrogen peroxide biosensor based on direct electrochemistry of hemoglobin in palladium nanoparticles/graphene-chitosan nanocomposite film. Appl Biochem Biotechnol 166(3):764–773. https://doi.org/10.1007/s12010-011-9465-y
Thema FT, Moloto MJ, Dikio ED, Nyangiwe NN, Kotsedi L, Maaza M, Khenfouch M (2013) Synthesis and characterization of graphene thin films by chemical reduction of exfoliated and intercalated graphite oxide. J Chemother 2013:6. https://doi.org/10.1155/2013/150536
Tian Y, Liu Y, Wang W, Zhang X, Peng W (2015) Sulfur-doped graphene-supported Ag nanoparticles for nonenzymatic hydrogen peroxide detection. J Nanopart Res 17(4):193. https://doi.org/10.1007/s11051-015-2976-7
Wang J (2008) Electrochemical glucose biosensors. Chem Rev 108(2):814–825. https://doi.org/10.1021/cr068123a
Wang H, Hao Q, Yang X, Lu L, Wang X (2010) Effect of graphene oxide on the properties of its composite with polyaniline. ACS Appl Mater Interfaces 2(3):821–828. https://doi.org/10.1021/am900815k
Wang Q-M, Niu H-L, Mao C-J, Song J-M, Zhang S-Y (2014) Facile synthesis of trilaminar core-shell Ag@C@Ag nanospheres and their application for H2O2 detection. Electrochim Acta 127:349–354. https://doi.org/10.1016/j.electacta.2014.02.051
Wang H, Bu Y, Dai W, Li K, Wang H, Zuo X (2015) Well-dispersed cobalt phthalocyanine nanorods on graphene for the electrochemical detection of hydrogen peroxide and glucose sensing. Sensors Actuators B Chem 216:298–306. https://doi.org/10.1016/j.snb.2015.04.044
Wang W, Ji Y, Zhang Y, Wang Z, Zhang T (2016) Green preparation of Au nanoparticles for electrochemical detection of H 2 O 2. J Semicond 37(1):013003
Xing L, Rong Q, Ma Z (2015) Non-enzymatic electrochemical sensing of hydrogen peroxide based on polypyrrole/platinum nanocomposites. Sensors Actuators B Chem 221:242–247. https://doi.org/10.1016/j.snb.2015.06.078
Xiong L, Zheng L, Xu J, Liu W, Kang X, Wang Y, . . . Xia J (2014) A non-enzyme hydrogen peroxide biosensor based on Fe3O4/RGO nanocomposite material. ECS Electrochem Lett 3(12): B26-B29. doi: https://doi.org/10.1149/2.0041412eel
Xu F, Deng M, Li G, Chen S, Wang L (2013) Electrochemical behavior of cuprous oxide–reduced graphene oxide nanocomposites and their application in nonenzymatic hydrogen peroxide sensing. Electrochim Acta 88:59–65. https://doi.org/10.1016/j.electacta.2012.10.070
Ye D, Luo L, Ding Y, Chen Q, Liu X (2011) A novel nitrite sensor based on graphene/polypyrrole/chitosan nanocomposite modified glassy carbon electrode. Analyst 136(21):4563–4569. https://doi.org/10.1039/C1AN15486A
Ye Y, Kong T, Yu X, Wu Y, Zhang K, Wang X (2012) Enhanced nonenzymatic hydrogen peroxide sensing with reduced graphene oxide/ferroferric oxide nanocomposites. Talanta 89:417–421. https://doi.org/10.1016/j.talanta.2011.12.054
Yu Y, Chen Z, He S, Zhang B, Li X, Yao M (2014) Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode. Biosens Bioelectron 52:147–152. https://doi.org/10.1016/j.bios.2013.08.043
Yu C, Wang L, Li W, Zhu C, Bao N, Gu H (2015) Detection of cellular H2O2 in living cells based on horseradish peroxidase at the interface of Au nanoparticles decorated graphene oxide. Sensors Actuators B Chem 211:17–24. https://doi.org/10.1016/j.snb.2015.01.064
Zhang Y, Pan C (2010) TiO2/graphene composite from thermal reaction of graphene oxide and its photocatalytic activity in visible light. J Mater Sci 46(8):2622–2626. https://doi.org/10.1007/s10853-010-5116-x
Zhang X, Liu D, Yu B, You T (2016) A novel nonenzymatic hydrogen peroxide sensor based on electrospun nitrogen-doped carbon nanoparticles-embedded carbon nanofibers film. Sensors Actuators B Chem 224:103–109. https://doi.org/10.1016/j.snb.2015.10.033
Zhang C, Jiang H, Ma R, Zhang Y, Chen Q (2017) Simple non-enzymatic electrochemical sensor for hydrogen peroxide based on nafion/platinum nanoparticles/reduced graphene oxide nanocomposite modified glassy carbon electrode. Ionics 23(5):1309–1317. https://doi.org/10.1007/s11581-016-1944-2
Zhao Y, Liu J, Hu Y, Cheng H, Hu C, Jiang C, . . . Qu L (2013) Highly compression-tolerant supercapacitor based on polypyrrole-mediated graphene foam electrodes. Adv Mater 25(4): 591–595. doi: https://doi.org/10.1002/adma.201203578
Zhao C, Zhang H, Zheng J (2017) A non-enzymatic electrochemical hydrogen peroxide sensor based on Ag decorated boehmite nanotubes/reduced graphene oxide nanocomposites. J Electroanal Chem 784:55–61. https://doi.org/10.1016/j.jelechem.2016.12.005
Zuo Y, Hoigne J (1993) Evidence for photochemical formation of H2O2 and oxidation of SO2 in authentic fog water. Science 260(5104):71–73. https://doi.org/10.1126/science.260.5104.71
Acknowledgements
M. H. N thanks the Higher Education Commission of Pakistan for startup research grants and the national research program for universities (21-329/SRGP/R&D/HEC/201 and 20-4993/R&D/HEC/14/614).
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Akhtar, M.A., Hayat, A., Iqbal, N. et al. Functionalized graphene oxide–polypyrrole–chitosan (fGO–PPy–CS) modified screen-printed electrodes for non-enzymatic hydrogen peroxide detection. J Nanopart Res 19, 334 (2017). https://doi.org/10.1007/s11051-017-4029-x
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DOI: https://doi.org/10.1007/s11051-017-4029-x