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A toxicity assessment of hydroxyapatite nanoparticles on development and behaviour of Drosophila melanogaster

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Abstract

The effects of oral intake of hydroxyapatite nanoparticles (HApNPs) were investigated on growth, development and behaviour of Drosophila. The Drosophila responses to various concentrations of HApNPs were compared. At lower concentrations, i.e. 5 mg L−1 more amount of oxidative stress was produced than that of highest concentration, i.e. 80 mg L−1. The increased amounts of oxidative stress reflect a higher amount of ROS production and increased cell damage within the larval gut. HApNPs was further shown to interfere with the calcium and phosphorus absorption pathway. Besides all these damage, HApNPs causes developmental delay in the late third instar larvae. The most significant anomaly was observed in pupae count, fly hatching after the feeding of HApNPs. Flies hatched from treated vials have decreased body weight with defective walking behaviour. Hatched flies have a phenotypic defect in the wing, eye and thorax of the bristles. Along with these changes, the adult fly becomes more prone towards stress. The findings hint that HApNPs persuade noxious effects and alter the development, structure, function and behaviour of the fly in a concentration-dependent manner.

Effect of Hydroxyapatite on the complete life cycle of Drosophila. Flies lay eggs in Hydroxyapatite containing food. As soon as the eggs hatch to larvae they start eating the NP contained food. The effect of Hydroxyapatite on various developmental stage is summerised by biochemical, immunohistochemical, behavioral, developmental and phenotypic defects.

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Acknowledgements

S.A. Pappus is thankful to DST-Inspire fellowship for the financial support he received to carry out this work. Two anonymous reviewers are thankfully acknowledged for their valuable comments, which help to improve the manuscript.

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  1. Department of Biological Sciences, IISER Kolkata, Haringhata Farm, Nadia, West Bengal, 741252, India

    S. Aurosman Pappus

  2. Department of Chemistry, National Institute of Technology, Rourkela, Odisha, 769008, India

    Basanti Ekka & Priyabrat Dash

  3. Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India

    Swetapadma Sahu, Debabrat Sabat & Monalisa Mishra

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  1. S. Aurosman Pappus
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Pappus, S.A., Ekka, B., Sahu, S. et al. A toxicity assessment of hydroxyapatite nanoparticles on development and behaviour of Drosophila melanogaster . J Nanopart Res 19, 136 (2017). https://doi.org/10.1007/s11051-017-3824-8

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