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The biological properties of the silver- and copper-doped ceramic biomaterial

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Abstract

The biological properties of nanostructured bioactive ceramic composite (BCC) granules doped with 0.1–10 at.% silver and 0.05–5 at.% copper have been investigated both in vitro and in vivo to develop effective alloplastic material for infected bone defect substitute. It is assumed that the granules consisting of biphasic calcium phosphate and bioactive glass ceramics due to their nanoscale (15–40 nm) and multiphase structure, bioelement placement in different ceramic phases as well as antimicrobial effect should improve osteogenic properties and biocompatibility. Tests in vitro have been conducted with multipotent mesenchymal stromal cells (MSCs) and test strains of microorganisms. The same biocomposite has been used in vivo to study the repair of bone defects in animal model. The findings indicate that doped BCC leads to antimicrobial activity. Inhibition of MSCs growth has been observed for granules doped with ions of more than 1 at.% silver and 0.5 at.% copper. The results of the in vivo study reveal that BCC implantation significantly improves bone reparation. Differences between bone repair with undoped and doped, with 1 at.% silver and 0.5 at.% copper, ceramic samples were not observed. The BCC doped within 0.5–1 at.% silver and 0.25–0.5 at.% copper stimulates bone tissue repair and has satisfactory biocompatibility and antimicrobial properties.

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Acknowledgments

The authors gratefully acknowledge the support of Dr. Olena Voloshuk from Bogomolets National Medical University (Ukraine), the Department of Microbiology, Virology, and Immunology. Dmytro Zubov, leading researcher and Roman Vasyliev, researcher from State Institute of Genetic and Regenerative Medicine of NAMS (Ukraine), in the implementation phases of the study. We express profound gratitude to Prof. Anatolii Levitskyi for the access to biochemical laboratory and vivarium facilities at State Institute of Stomatology of NAMS (Odesa, Ukraine). This project is financially supported by Branch target preparation Kyiv National Taras Shevchenko University, NAMS of Ukraine (Grant No. 0114U003876).

Conflict of interest

The authors have no financial interest in any of the companies or products, devices, and biomedical preparation mentioned in this manuscript. Therefore, authors have no conflict of interests.

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Authors and Affiliations

  1. Department of Therapeutic Stomatology, Bogomolets National Medical University, 34 Peremohy Av., Kyiv, 01601, Ukraine

    Oleksandr Lysenko & Anatolii Borysenko

  2. Department of Analytical Chemistry and Functional Ceramics, Institute for Problems of Material Science NASU, 3 Krzhizhanovsky Str., Kyiv, 03142, Ukraine

    Oleksii Dubok & Oleksandr Shinkaruk

  3. Department of Inorganic Chemistry, Faculty of Chemistry, National Taras Shevchenko University of Kyiv, 64 Volodymyrska Str., Kyiv, 01601, Ukraine

    Oleksii Dubok

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  1. Oleksandr Lysenko
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  2. Oleksii Dubok
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  3. Anatolii Borysenko
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  4. Oleksandr Shinkaruk
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Correspondence to Oleksandr Lysenko.

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Guest Editor: Liudmyla Rieznichenko

This article is part of the topical collection on Engineered Bioinspired Nanomaterials

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Lysenko, O., Dubok, O., Borysenko, A. et al. The biological properties of the silver- and copper-doped ceramic biomaterial. J Nanopart Res 17, 178 (2015). https://doi.org/10.1007/s11051-015-2971-z

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