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An investigation of in vivo wound healing activity of biologically synthesized silver nanoparticles

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Abstract

Therapeutic use of nano-silver is claimed to have reduced side effects and enhanced curative activity as compared to its ionic counterpart (silver ions). The present work aims to screen microbes for the synthesis of silver nanoparticles (AgNPs), to formulate the nano-silver-based Carbopol gel and evaluating its wound healing efficacy on rat model. The goal was to develop the topical formulation based on bio-nano-silver to control the infection and healing the wounds with higher efficacy. Procedure involved the use of Saccharomyces boulardii for the synthesis of silver nanoparticles in the size range of 3–10 nm and these nanoparticles were used for the preparation of Carbopol-based nano-silver gel. Highly stable Carbopol nanogel was developed with good rheological properties. The burn wound healing potential of this nano-silver gel was evaluated on SD rats via visual observation, transepidermal water loss and histology of skin. Excellent wound healing was observed with AgNPs. Biologically synthesized AgNPs-based nano-silver gel showed superior wound healing efficacy as compared to marketed formulations and silver ions.

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Acknowledgments

Authors Abhishek Kaler and Harshad Harde are thankful to the Department of Biotechnology, Government of India, for providing research fellowships. Amit Kumar Mittal acknowledges the CSIR, and Ashish Agrawal wishes to thank DST, Government of India, for providing research fellowship. The authors also gratefully acknowledge Dr. Jayeeta Bhaumik for critically going through the manuscript.

Conflict of interest

None of the authors have a financial interest in any of the products, devices, or drugs mentioned in this manuscript.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, 160062, Punjab, India

    Abhishek Kaler, Amit Kumar Mittal & Uttam Chand Banerjee

  2. Department of Pharmaceutics, Centre for Pharmaceutical Nanotechnology, National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, 160062, Punjab, India

    Mahesh Katariya, Harshad Harde, Ashish Kumar Agrawal & Sanyog Jain

Authors
  1. Abhishek Kaler
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  2. Amit Kumar Mittal
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  3. Mahesh Katariya
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  4. Harshad Harde
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  5. Ashish Kumar Agrawal
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  6. Sanyog Jain
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  7. Uttam Chand Banerjee
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Corresponding author

Correspondence to Uttam Chand Banerjee.

Additional information

Mahesh Katariya, Harshad Harde and Ashish Kumar Agrawal have contributed equally to the manuscript.

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Kaler, A., Mittal, A.K., Katariya, M. et al. An investigation of in vivo wound healing activity of biologically synthesized silver nanoparticles. J Nanopart Res 16, 2605 (2014). https://doi.org/10.1007/s11051-014-2605-x

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  • DOI: https://doi.org/10.1007/s11051-014-2605-x

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