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In vitro comparative study of pure hydroxyapatite nanorods and novel polyethylene glycol/graphene oxide/hydroxyapatite nanocomposite

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Abstract

In this work, crystalline hydroxyapatite (HAP) nanorods were first prepared by a simple precipitation method in the presence of a new capping agent based on Schiff base compounds, and then composite of polyethylene glycol (PEG), graphene oxide (GO), and the HAP nanorods was synthesized by freeze-drying method. X-ray diffraction patterns indicated that poor crystalline HAP powders produced in the absence of Schiff base were highly agglomerated. In addition, in vitro bioactivity of the produced HAP nanorods and PEG/GO/HAP nanocomposite was evaluated by soaking the products in simulated body fluid (SBF). The chemical composition of the SBF solutions was analyzed by inductively coupled plasma-optical emission spectrometry, and it was found that more Ca and P were released from the nanocomposite compared to the pure HAP nanorods, indicating high bioactivity of the nanocomposite. In addition, it was observed that the growth of new apatite on the surface of the nano-sized materials increased after immersion for 14 days in the SBF solution.

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References

  • Babaei Z, Jahanshahi M, Rabiee SM (2013) The fabrication of nanocomposites via calcium phosphate formation on gelatin-chitosan network and the gelatin influence on the properties of biphasic composites. Mater Sci Eng C 33:370–375

    Article  Google Scholar 

  • Das TK, Prusty S (2012) Graphene: a revolution in nanobiotechnology. J Res Nanobiotechnol 1:019–030

    Google Scholar 

  • Deen I, Pang X, Zhitomirsky I (2012) Electrophoretic deposition of composite chitosan-halloysite nanotube-hydroxyapatite films. Colloids Surf A Physicochem Eng Asp 410:38–44

    Article  Google Scholar 

  • Dhanalakshmi CP, Vijayalakshmi L, Narayanan V (2012) Synthesis and preliminary characterization of polyethylene glycol (PEG)/hydroxyapatite (HAp) nanocomposite for biomedical applications. Int J Phys Sci 7:2093–2101

    Article  Google Scholar 

  • Hench LL (1991) Bioceramics: from concept to clinic. J Am Ceram Soc 74:1487–1510

    Article  Google Scholar 

  • Hummers WS, Offeman RE (1958) Preparation of graphitic oxide. J Am Chem Soc 80:1339

    Article  Google Scholar 

  • Itokawa H, Hiraide T, Moriya M, Fujimoto M, Nagashima G, Suzuki R, Fujimoto T (2007) A 12 month in vivo study on the response of bone to a hydroxyapatite-polymethylmethacrylate cranioplasty composite. Biomaterials 28:4922–4927

    Article  Google Scholar 

  • Jarcho M (1981) Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop Relat Res 157:259–278

    Google Scholar 

  • Jiao TF, Zhou J, Zhou JX, Gao LH, Xing YY, Li XH (2011) Synthesis and characterization of chitosan-based Schiff base compounds with aromatic substituent groups. Iran Poly J 20:123–126

    Google Scholar 

  • Joshi SV, Srivastava MP, Pal A, Pal S (1993) Plasma spraying of biologically derived hydroxyapatite on implantable materials. J Mater Sci Mater Med 4:251–255

    Article  Google Scholar 

  • Kaflak A, Slósarczyk A, Kolodziejski W (2011) A comparative study of carbonate bands from nanocrystalline carbonated hydroxyapatites using FT-IR spectroscopy in the transmission and photoacoustic modes. J Mol Struct 997:7–17

    Article  Google Scholar 

  • Kasuga T, Ota Y, Nogami M, Abe Y (2000) Preparation and mechanical properties of polylactic acid composites containing hydroxyapatite fibers. Biomaterials 22:19–23

    Article  Google Scholar 

  • Kheradmandfar M, Fathi MH, Ahangarian M, Zahrani EM (2012) In vitro bioactivity evaluation of magnesium-substituted fluorapatite nanopowders. Ceram Int 38:169–175

    Article  Google Scholar 

  • Kim H, Namgung R, Singha K, Oh IK, Kim WJ (2011) Graphene oxide-polyethylenimine nanoconstruct as a gene delivery vector and bioimaging tool. Bioconjugate Chem 22:2558–2567

    Article  Google Scholar 

  • Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 27:2907–2915

    Article  Google Scholar 

  • Li P, Ohtsuki C, Kokubo T (1994) The role of hydrated silica, Titania, and alumina in inducing apatite on implants. J Biomed Mater Res 28:7–15

    Article  Google Scholar 

  • Li D, Muller MB, Gilje S, Kaner RB, Wallace GG (2008) Processable aqueous dispersions of graphene nanosheets. Nat Nanotechnol 3:101–105

    Article  Google Scholar 

  • Li M, Wang Y, Liu Q, Li Q, Cheng Y, Zheng Y, Xi T, Wei S (2013) In situ synthesis and biocompatibility of nano hydroxyapatite on pristine and chitosan functionalized graphene oxide. J Mater Chem B 1:475–484

    Article  Google Scholar 

  • Liu H, Xi P, Xie G, Shi Y, Hou F, Huang L, Chen F, Zeng Z, Shao C, Wang J (2012) Simultaneous reduction and surface functionalization of graphene oxide for hydroxyapatite mineralization. J Phys Chem C 116:3334–3341

    Article  Google Scholar 

  • Marcano DC, Kosynkin DV, Berlin JM, Sinitskii A, Sun Z, Slesarev A, Alemany LB, Lu W, Tour JM (2010) Improved synthesis of graphene oxide. ACS Nano 4:4806–4814

    Article  Google Scholar 

  • Marques P, Gonçalves G, Singh MK, Grácio J (2012) Graphene oxide and hydroxyapatite as fillers of polylactic acid nanocomposites: preparation and characterization. J Nanosci Nanotechnol 12:6686–6692

    Article  Google Scholar 

  • Murugan R, Ramakrishna S (2004) Bioresorbable composite bone paste using polysaccharide based nano hydroxyapatite. Biomaterials 25:3829–3835

    Article  Google Scholar 

  • Ni JH, Shi YL, Yan FY, Chen JZ, Wang L (2008) Preparation of hydroxyapatite-containing titania coating on titanium substrate by micro-arc oxidation. Mater Res Bull 43:45–53

    Article  Google Scholar 

  • Novoselov KS, Geim AK, Morozov SV, Jiang D, Zhang Y, Dubonos SV, Grigorieva IV, Firsov AA (2004) Electric field effect in atomically thin carbon films. Science 306:666–669

    Article  Google Scholar 

  • Polu AR, Kumar R, Causin V, Neppalli R (2011) Conductivity, XRD, and FTIR studies of new Mg2+ -ion-conducting solid polymer electrolytes: [PEG: Mg(CH3COO)2]. J Korean Phys Soc 59:114–118

    Article  Google Scholar 

  • Rodriguez K, Renneckar S, Gatenholm P (2011) Biomimetic calcium phosphate crystal mineralization on electrospun cellulose based scaffolds. ACS Appl Mater Interface 3:681–689

    Article  Google Scholar 

  • Sadat-Shojai M, Khorasani MT, Jamshidi A (2012) Hydrothermal processing of hydroxyapatite nanoparticles-A Taguchi experimental design approach. J Cryst Growth 361:73–84

    Article  Google Scholar 

  • Spanos N, Misirlis DY, Kanellopoulou DG, Koutsoukos PG (2006) Seeded growth of hydroxyapatite in simulated body fluid. J Mater Sci 41:1805–1812

    Article  Google Scholar 

  • Tavakoli F, Salavati-Niasari M (2014) A facile synthesis of CuI-graphene nanocomposite by glucose as a green capping agent and reductant. J Ind Eng Chem. doi:10.1016/j.jiec.2013.11.061

  • Venkatesan J, Qian ZJ, Ryu B, Kumar NA, Kim SK (2011) Preparation and characterization of carbon nanotube-grafted-chitosan-natural hydroxyapatite composite for bone tissue engineering. Carbohydr Poly 83:569–577

    Article  Google Scholar 

  • Wahl DA, Czernuszka JT (2006) Collagen-hydroxyapatite composites for hard tissue repair. Euro Cells Mater 11:43–56

    Google Scholar 

  • Wakeland S, Martinez R, Grey JK, Luhrs CC (2010) Production of graphene from graphite oxide using urea as expansion-reduction agent. Carbon 48:3463–3470

    Article  Google Scholar 

  • White AA, Best SM, Kinloch IA (2007) Hydroxyapatite-carbon nanotube composites for biomedical applications: a review. Int J Appl Ceram Technol 4:1–13

    Article  Google Scholar 

  • With GD, Van Dijk HJA, Hattu N, Prijs K (1981) Preparation, micro-structure and mechanical properties of dense polycrystalline hydroxyapatite. J Mater Sci 16:1592–1598

    Article  Google Scholar 

  • Wu G, Su B, Zhang W, Wang C (2008) In vitro behaviors of hydroxyapatite reinforced polyvinyl alcohol hydrogel composite. Mater Chem Phys 107:364–369

    Article  Google Scholar 

  • Yang K, Zhang S, Zhang G, Sun X, Lee ST, Liu Z (2010) Graphene in mice: Ultrahigh in vivo tumor uptake and efficient photothermal therapy. Nano Lett 10:3318–3323

    Article  Google Scholar 

  • Yang XY, Wang YS, Huang X (2011) Multi-functionalized graphene oxide based anticancer drug-carrier with dual-targeting function and pH sensitivity. J Mater Chem 21:3448–3454

    Article  Google Scholar 

  • Yu CC, Chang JJ, Lee YH, Lin YC, Wu MH, Yang MC, Chien C-T (2013) Electrospun scaffolds composing of alginate, chitosan, collagen and hydroxyapatite for applying in bone tissue engineering. Mater Lett 93:133–136

    Article  Google Scholar 

  • Zhang Q, Chen J, Feng J, Cao Y, Deng C, Zhang X (2003) Dissolution and mineralization behaviors of HA coatings. Biomaterials 24:4741–4748

    Article  Google Scholar 

  • Zhang S, Xiong P, Yang X, Wang X (2011) Novel PEG functionalized graphene nanosheets: enhancement of dispersibility and thermal stability. Nanoscale 3:2169–2174

    Article  Google Scholar 

  • Zhao B, Hu H, Mandal SK, Haddon RC (2005) A bone mimic based on the self-assembly of hydroxyapatite on chemically functionalized single-walled carbon nanotubes. Chem Mater 17:3235–3241

    Article  Google Scholar 

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Acknowledgments

Authors are grateful to council of University of Kashan for providing financial support to undertake this work.

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Authors and Affiliations

  1. Department of Inorganic Chemistry, Faculty of Chemistry, University of Kashan, P. O. Box. 87317-51167, Kashan, Islamic Republic of Iran

    Fatemeh Mohandes & Masoud Salavati-Niasari

  2. Institute of Nano Science and Nano Technology, University of Kashan, P. O. Box. 87317-51167, Kashan, Islamic Republic of Iran

    Masoud Salavati-Niasari

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  1. Fatemeh Mohandes
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  2. Masoud Salavati-Niasari
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Correspondence to Masoud Salavati-Niasari.

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Mohandes, F., Salavati-Niasari, M. In vitro comparative study of pure hydroxyapatite nanorods and novel polyethylene glycol/graphene oxide/hydroxyapatite nanocomposite. J Nanopart Res 16, 2604 (2014). https://doi.org/10.1007/s11051-014-2604-y

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