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Enzyme-encapsulated silica nanoparticle for cancer chemotherapy

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Abstract

A novel horseradish peroxidase-encapsulated silica nanoparticle (SNP) was generated in this study under relatively mild conditions. The generated enzyme-encapsulated SNP were relatively uniform in size (average 70 ± 14.3 nm), monodispersed, and spherical, as characterized by transmission electron microscopy and scanning electron microscopy. The horseradish peroxidase encapsulated in silica nanoparticle exhibits biological properties, such as a pH-dependent activity profile and k m value, similar to that of free enzymes. Furthermore, enzyme-encapsulated SNP exhibited good operational stability for the repetitive usage with a relative standard deviation of 5.1 % (n = 10) and a high stability for long term storage (>60 days) at 4 °C. The feasibility of using enzyme-encapsulated SNP in prodrug cancer therapy was also demonstrated by its capability to convert the prodrug indole-3-acetic acid into cytotoxic peroxyl radicals and trigger the death of tumor cells. These results indicate that the developed enzyme-encapsulated SNP has potential in the applications of prodrug cancer therapy.

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Acknowledgments

The authors would like to thank Dr. Hoi-Ping Shi and the laboratory of Dr. Tung-Kung Wu for technical support. The authors would like to thank the ATU plan of Ministry of Education of the R.O.C. (Taiwan) and the National Science Council of the R.O.C. (Taiwan) under Contract No. NSC 97-2311-B-009-001-MY3 for financially supporting this research.

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Authors and Affiliations

  1. Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan, ROC

    Yi-Rong Chiu, Wei-Jen Ho, Jiun-Shuan Chao & Chiun-Jye Yuan

  2. Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, 300, Taiwan, ROC

    Chiun-Jye Yuan

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  1. Yi-Rong Chiu
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  2. Wei-Jen Ho
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  3. Jiun-Shuan Chao
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Correspondence to Chiun-Jye Yuan.

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Y.-R. Chiu and W.-J. Ho contributed equally to this work.

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Chiu, YR., Ho, WJ., Chao, JS. et al. Enzyme-encapsulated silica nanoparticle for cancer chemotherapy. J Nanopart Res 14, 829 (2012). https://doi.org/10.1007/s11051-012-0829-1

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