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Mesoporous silica-coated superparamagnetic particles prepared by pseudomorphic transformation and their application in purification of plasmid DNA

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Abstract

Mesoporous silica-coated superparamagnetic particles were prepared via pseudomorphic transformation of pre-made amorphous silica-coated Fe3O4–polymer composite particles using n-cetyltrimethylammonium bromide (CTAB) and 1,3,5-trimethyl benzene (MES) as template. The mesoporous particles presented almost the same size, shape, and magnetic property as the original amorphous particles but an ordered mesoporous shell with wormhole-like pore structure. The pore size of the shells increased from 2.4 to 3.1 and 4.2 nm as the molar ratio of MES/CTAB increased from 0 to 1.0 and 1.5. DNA extraction experiments showed the mesoporous particles were qualified for purification of plasmid DNA from bacterial lysate.

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Acknowledgment

This study was supported by National Basic Research Program of China (No. 2011CB935800) and the National Nature Science Foundation of China (No. 51072064, 50825202).

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Authors and Affiliations

  1. State Key Laboratory of Supramolecular Structures and Materials, College of Chemistry, Jilin University, Changchun, 130012, People’s Republic of China

    Feng Yan, Lin Sun, Jiaqi Zhuang & Wensheng Yang

  2. Department of Andrology, The First Hospital, Jilin University, Changchun, 130021, People’s Republic of China

    Fubiao Li & Hongliang Wang

  3. College of Chemistry and Resources Environment, Linyi Normal University, Linyi, 276005, People’s Republic of China

    Feng Yan

Authors
  1. Feng Yan
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  2. Lin Sun
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  3. Fubiao Li
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  4. Jiaqi Zhuang
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  5. Hongliang Wang
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  6. Wensheng Yang
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Correspondence to Hongliang Wang or Wensheng Yang.

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Yan, F., Sun, L., Li, F. et al. Mesoporous silica-coated superparamagnetic particles prepared by pseudomorphic transformation and their application in purification of plasmid DNA. J Nanopart Res 13, 6613–6620 (2011). https://doi.org/10.1007/s11051-011-0569-7

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  • DOI: https://doi.org/10.1007/s11051-011-0569-7

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