Skip to main content

Advertisement

SpringerLink
Log in

Silver nanoparticles with gelatin nanoshells: photochemical facile green synthesis and their antimicrobial activity

  • Research paper
  • Published:
Journal of Nanoparticle Research Aims and scope Submit manuscript

Abstract

In the current study, a facile green synthesis of silver-gelatin core–shell nanostructures (spherical, spherical/cubic hybrid, and cubic, DLS diameter: 4.1–6.9 nm) is reported via the wet chemical synthesis procedure. Sunlight-UV as an available reducing agent cause mild reduction of silver ions into the silver nanoparticles (Ag-NPs). Gelatin protein, as an effective capping/shaping agent, was used in the reaction to self-assemble silver nanostructures. The formation of silver nanostructures and their self-assembly pattern was confirmed by SEM, AFM, and TEM techniques. Further investigations were carried out using zeta-potential, UV–Vis, FTIR, GPC, and TGA/DTG/DTA data. The prepared Ag-NPs showed proper and acceptable antimicrobial activity against three classes of microorganisms (Escherichia coli Gram-negative bacteria, Staphylococcus aureus Gram-positive bacteria, and Candida albicans fungus). The antibacterial and antifungal Ag-NPs exhibit good stability in solution and can be considered as promising candidates for a wide range of biomedical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Scheme 2
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • AbuBakar NHH, Ismail J, AbuBakar M (2007) Synthesis and characterization of silver nanoparticles in natural rubber. Mater Chem Phys 104:276–283

    Article  CAS  Google Scholar 

  • AshaRani PV, Hande MP, Valiyaveettil S (2009) Anti-proliferative activity of silver nanoparticles. BMC Cell Biol 10:1–14

    Article  Google Scholar 

  • Bale SS, Asuri P, Karajanagi SS, Dordick JS, Kane RS (2007) Protein-directed formation of silver nanoparticles on carbon nanotubes. Adv Mater 19:3167–3170

    Article  CAS  Google Scholar 

  • Burt J, Gutierrez-Wing C, Miki-Yoshida M, Jose-Yacaman M (2004) Noble-metal nanoparticles directly conjugated to globular proteins. Langmuir 20:11778–11783

    Article  CAS  Google Scholar 

  • Chen J, Wang J, Zhang X, Jin Y (2008) Microwave assisted green synthesis of silver nanoparticles by carboxymethyl cellulose sodium and silver nitrate. Mater Chem Phys 108:421–424

    Article  CAS  Google Scholar 

  • Feng QL, Wo J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52:662–668

    Article  CAS  Google Scholar 

  • Filippo E, Serra A, Manno D (2009) Poly (vinyl alcohol) capped silver nanoparticles as localized surface Plasmon resonance-based hydrogen peroxide. Sens Actuators B Chem 138:625–630

    Article  Google Scholar 

  • Grzelczak M, Vermant J, Furst EM, Liz-Marzan LM (2010) Directed self-assembly of nanoparticles. ACS Nano 4:3591–3605

    Article  CAS  Google Scholar 

  • Gurunathan S, Lee KJ, Kalishwaralal K, Sheikpranbabu S, Vaidyanathan R, Eom SH (2009) Antiangiogenic properties of silver nanoparticles. Biomaterials 30:6341–6350

    Article  CAS  Google Scholar 

  • Jaiswal S, Duffy B, Jaiswal AK, Stobie N, McHale P (2010) Enhancement of the antibacterial properties of silver nanoparticles using β-cyclodextrin as a capping agent. Int J Antimicrob Ag 36:280–283

    Article  CAS  Google Scholar 

  • Janardhanan R, Karuppaiah M, Hebalkar N, Rao TN (2009) Synthesis and surface chemistry of nano silver particles. Polyhedron 28:2522–2530

    Article  CAS  Google Scholar 

  • Kassaee MZ, Akhavan A, Sheikh N, Beteshobabrud R (2008) γ-ray synthesis of starch-stabilized silver nanoparticles with antibacterial activities. Radiat Phys Chem 77:1074–1078

    Article  CAS  Google Scholar 

  • Kim KJ, Sung WS, Suh BK, Moon SK, Choi JS, Kim JG, Lee DG (2009) Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals 22:235–242

    Article  CAS  Google Scholar 

  • Ko S, Park TJ, Kim HS, Kim JH, Cho YJ (2009) Directed self-assembly of gold binding polypeptide-protein A fusion proteins for development of gold nanoparticle-based SPR immunosensors. Biosens Bioelectron 24:2592–2597

    Article  CAS  Google Scholar 

  • Lara HH, Ayala-Nunez NV, Ixtepan-Turrent L, Rodriguez-Padilla C (2010) Mode of antiviral action of silver nanoparticles against HIV-1. J. Nanobiotechnology 8. http://www.jnanobiotechnology.com/content/8/1/1

  • Lee JS, Lytton-Jean AKR, Hurst SJ, Mirkin CA (2007) Silver nanoparticle-oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties. Nano Lett 7:2112–2115

    Article  CAS  Google Scholar 

  • Leung TC, Wong CK, Xie Y (2010) Green synthesis of silver nanoparticles using biopolymers, carboxymethylated-curdlan and fucoidan. Mater Chem Phys 121:402–405

    Article  CAS  Google Scholar 

  • Li WR, Xie XB, Shi QS, Zeng HY, Yang YSO, Chen YB (2010) Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol 85:1115–1122

    Article  CAS  Google Scholar 

  • Liu X, Zhang F, Huang R, Pan C, Zhu J (2008) Capping modes in pvp-directed silver nanocrystal growth: multi-twinned nanorods versus single-crystalline nano-hexapods. Cryst Growth Des 8:1916–1923

    Article  CAS  Google Scholar 

  • Liu Y, Chen S, Zhong L, Wu G (2009) Preparation of high-stable silver nanoparticle dispersion by using sodium alginate as a stabilizer under gamma irradiation. Radiat Phys Chem 78:251–255

    Article  CAS  Google Scholar 

  • Liu Y, Liu X, Wang X, Yang J, Yang X, Lu L (2010) Gelatin-g-poly (methyl methacrylate)/silver nanoparticle hybrid films and the evaluation of their antibacterial activity. J Appl Polym Sci 116:2617–2625

    Article  CAS  Google Scholar 

  • Lu X, Cui S (2010) Wool keratin-stabilized silver nanoparticles. Bioresour Technol 101:4703–4707

    Article  Google Scholar 

  • Medina-Ramirez I, Bashir S, Luo Z, Liu JL (2009) Green synthesis and characterization of polymer-stabilized silver nanoparticles. Colloid Surface B73:185–191

    Google Scholar 

  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–2353

    Article  CAS  Google Scholar 

  • Nadworny PL, Wang J, Tredget EE, Burrell RE (2008) Anti-inflammatory activity of nanocrystalline silver in a porcine contact dermatitis model. Nanomedicine 4:241–251

    Article  CAS  Google Scholar 

  • Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73:1712–1720

    Article  CAS  Google Scholar 

  • Panacek A, Kolar M, Vecerova R, Prucek R, Soukupov J, Krystof V, Hamal P, Zboril R, Kvıtek L (2009) Antifungal activity of silver nanoparticles against Candida spp. Biomaterials 30:6333–6340

    Article  CAS  Google Scholar 

  • Philip D (2010) Honey mediated green synthesis of silver nanoparticles. Spectrochim Acta A75:1078–1081

    Google Scholar 

  • Pinto VV, Ferreira MJ, Silva R, Santos HA, Silva F, Pereira CM (2010) Long time effect on the stability of silver nanoparticles in aqueous medium: effect of the synthesis and storage conditions. Colloids Surf A Physicochem Eng Asp 364:19–25

    Article  CAS  Google Scholar 

  • Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83

    Article  CAS  Google Scholar 

  • Rao YN, Banerjee D, Datta A, Das SK, Guin R, Saha A (2010) Gamma irradiation route to synthesis of highly re-dispersible natural polymer capped silver nanoparticles. Radiat Phys Chem 79:1240–1246

    Article  CAS  Google Scholar 

  • Salgado EN, Lewis RA, Faraone-Mennella J, Tezcan FA (2008) Metal-mediated self-assembly of protein superstructures: influence of secondary interactions on protein oligomerization and aggregation. J Am Chem Soc 130:6082–6608

    Article  CAS  Google Scholar 

  • Salgado EN, Radford RJ, Tezcan FA (2010) Metal-directed protein self-assembly. Acc Chem Res 43:661–672

    Article  CAS  Google Scholar 

  • Sambhy V, MacBride MM, Peterson BR, Sen A (2006) Silver bromide nanoparticle/polymer composites: dual action tunable antimicrobial materials. J Am Chem Soc 128:9798–9808

    Article  CAS  Google Scholar 

  • Sanghi R, Verma P (2009) Biomimetic synthesis and characterisation of protein capped silver nanoparticles. Bioresour Technol 100:501–504

    Article  CAS  Google Scholar 

  • Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145:83–96

    Article  CAS  Google Scholar 

  • Shrivastava S, Bera T, Singh SK, Singh G, Ramachandrarao P, Dash D (2009) Characterization of anti-platelet properties of silver nanoparticles. ACS Nano 3:1357–1364

    Article  CAS  Google Scholar 

  • Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 275:177–182

    Article  CAS  Google Scholar 

  • Tilaki RM, Iraji-zad A, Mahdavi SM (2006) Stability, size and optical properties of silver nanoparticles prepared by laser ablation in different carrier media. Appl Phys A84:215–219

    Google Scholar 

  • Vaidyanathan R, Kalishwaralal K, Gopalram S, Gurunathan S (2009) Nanosilver—the burgeoning therapeutic molecule and its green synthesis. Biotechnol Adv 27:924–937

    Article  CAS  Google Scholar 

  • Veerapandian M, Lim SK, Nam HM, Kuppannan G, Yun KS (2010) Glucosamine-functionalized silver glyconanoparticles: characterization and antibacterial activity. Anal Bioanal Chem 398:867–876

    Article  CAS  Google Scholar 

  • Wei D, Sun W, Qian W, Ye Y, Ma X (2009) The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohyd Res 344:2375–2382

    Article  CAS  Google Scholar 

  • Xu X, Zhou M (2008) Antimicrobial gelatin nanofibers containing silver nanoparticles. Fiber Polym 9:685–690

    Article  CAS  Google Scholar 

  • Zhang J, Geddes CD, Lakowicz JR (2004) Complexation of polysaccharide and monosaccharide with thiolate boronic acid capped on silver nanoparticle. Anal Biochem 332:253–260

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. G. R. Bardajee, Dr. M. Adeli, Mr. H. Rezanejad, Mr. H. Abroshan, Mr. M. Akhlaghi, and Mr. M. Fakourpour for their valuable help in preparation of this article, making the samples, and data taking.

Author information

Authors and Affiliations

  1. Polymer Research Laboratory, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran

    Ali Pourjavadi & Rouhollah Soleyman

Authors
  1. Ali Pourjavadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rouhollah Soleyman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ali Pourjavadi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pourjavadi, A., Soleyman, R. Silver nanoparticles with gelatin nanoshells: photochemical facile green synthesis and their antimicrobial activity. J Nanopart Res 13, 4647–4658 (2011). https://doi.org/10.1007/s11051-011-0428-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11051-011-0428-6

Keywords

Search

Navigation