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Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

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Abstract

Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis.

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Acknowledgment

This study is supported by Walloon Region (Targan Project, grant 516071). C Michiels is a Research Associate of the Belgian FNRS.

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Authors and Affiliations

  1. Drug Design and Discovery Center, FUNDP, University of Namur, Namur, Belgium

    Bernard Masereel, Mustapha Dinguizli & Nicolas Moniotte

  2. Pole of Pharmacology and Therapeutics, University of Louvain, Louvain, Belgium

    Caroline Bouzin, Olivier Feron & Bernard Gallez

  3. Mont-Godinne Hospital, University of Louvain, Louvain, Belgium

    Thierry Vander Borght

  4. Department of Biology, FUNDP, University of Namur, Namur, Belgium

    Carine Michiels

  5. Research Center in Physics of Matter and Radiation, FUNDP, University of Namur, Namur, Belgium

    Stéphane Lucas

Authors
  1. Bernard Masereel
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  2. Mustapha Dinguizli
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  3. Caroline Bouzin
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  4. Nicolas Moniotte
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  5. Olivier Feron
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  6. Bernard Gallez
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  7. Thierry Vander Borght
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  8. Carine Michiels
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  9. Stéphane Lucas
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Correspondence to Bernard Masereel.

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Masereel, B., Dinguizli, M., Bouzin, C. et al. Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes. J Nanopart Res 13, 1573–1580 (2011). https://doi.org/10.1007/s11051-010-9908-3

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  • DOI: https://doi.org/10.1007/s11051-010-9908-3

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