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Inhibitory Effects of Photodynamic Inactivation on Planktonic Cells and Biofilms of Candida auris

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Abstract

Candida auris is an emerging pathogen that has caused numerous severe infections in recent years, and has therefore become a global concern for public health agencies. Most conventional antifungal agents, especially fluconazole, have shown limited effects on this pathogen. New methods to restrict this pathogen are in urgent demand. Antimicrobial photodynamic therapy (aPDT) has been shown to be a promising technique against multiple pathogenic fungi. This study sought to determine the in vitro effect of aPDT using methylene blue (MB) combined with light-emitting diode (LED) on the viability of planktonic cells and biofilms of five clinical strains of C. auris. MB (8, 16 and 32 μg/ml) was applied as the photosensitizer, and a LED (635 nm, 12 and 24 J/cm2) device was used as light source to activate the photosensitizer. The results showed that there was no growth of tested C. auris strains following aPDT on planktonic cultures. In addition, aPDT exhibited colony-forming unit reduction of up to 7.20 log10 against C. auris biofilms. These data demonstrate that in vitro aPDT with MB and LED offers promising potential for the treatment of C. auris infections.

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Acknowledgements

We gratefully acknowledge Professor Haoping Liu from University of California, Irvine, for kindly providing us with isolates studied.

Funding

This work was supported by National Natural Science Foundation of China (31400131 to Lujuan Gao and 81401677 to Yi Sun), Natural Science Foundation of Shanghai (16ZR1431300 to Jingwen Tan), Shanghai Municipal Commission of Health and Family Planning (20154Y0196 to Jingwen Tan) and Fundamental Research Funds for the Central Universities (22120180341 to Jingwen Tan).

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Author notes

  1. Jingwen Tan and Zhaoyang Liu contributed equally to this study and are joint first authors.

Authors and Affiliations

  1. Department of Medical Mycology, Shanghai Dermatology Hospital, Shanghai, 200443, China

    Jingwen Tan & Lianjuan Yang

  2. Department of Stomatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, 434100, China

    Zhaoyang Liu

  3. Department of Dermatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, 434100, China

    Yi Sun

  4. Department of Dermatology, Zhongshan Hospital Fudan University, Shanghai, 200032, China

    Lujuan Gao

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  1. Jingwen Tan
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Correspondence to Lianjuan Yang or Lujuan Gao.

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Tan, J., Liu, Z., Sun, Y. et al. Inhibitory Effects of Photodynamic Inactivation on Planktonic Cells and Biofilms of Candida auris. Mycopathologia 184, 525–531 (2019). https://doi.org/10.1007/s11046-019-00352-9

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  • DOI: https://doi.org/10.1007/s11046-019-00352-9

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