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Effects of Antifungal Agents Alone and in Combination Against Candida glabrata Strains Susceptible or Resistant to Fluconazole

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Abstract

The rise of Candida spp. resistant to classic triazole antifungal agents has led to a search for new therapeutic options. Here, we evaluated combinations of antifungals in a checkerboard assay against two groups of Candida glabrata strains: one containing fluconazole-susceptible clinical isolates (FS) and another containing fluconazole-resistant laboratory derivative (FR). The most synergistic combination observed was amphotericin B + flucytosine (synergistic for 61.77 % of FS strains and 76.47 % of FR strains). The most antagonistic combination observed was ketoconazole + flucytosine (FS 61.77 % and FR 55.88 %). Surprisingly, most combinations evidenced indifferent interactions, and the best synergism appeared when amphotericin B and flucytosine were combined against both groups of isolates.

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Authors and Affiliations

  1. Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Rua Andradas 1985/201, Santa Maria, RS, 97010-033, Brazil

    Izabel Almeida Alves, Laíssa Arévalo Bandeira, Débora Alves Nunes Mario, Laura Bedin Denardi, Louise Vignoles Neves, Janio Morais Santurio & Sydney Hartz Alves

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  1. Izabel Almeida Alves
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  2. Laíssa Arévalo Bandeira
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Correspondence to Débora Alves Nunes Mario.

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Alves, I.A., Bandeira, L.A., Mario, D.A.N. et al. Effects of Antifungal Agents Alone and in Combination Against Candida glabrata Strains Susceptible or Resistant to Fluconazole. Mycopathologia 174, 215–221 (2012). https://doi.org/10.1007/s11046-012-9538-7

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