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Variation in Genotype and Higher Virulence of a Strain of Sporothrix schenckii Causing Disseminated Cutaneous Sporotrichosis

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Abstract

Sporotrichosis is usually a localized, lymphocutaneous disease, but its disseminated type was rarely reported. The main objective of this study was to identify specific DNA sequence variation and virulence of a strain of Sporothrix schenckii isolated from the lesion of disseminated cutaneous sporotrichosis. We confirmed this strain to be S. schenckii by® tubulin and chitin synthase gene sequence analysis in addition to the routine mycological and partial ITS and NTS sequencing. We found a 10-bp deletion in the ribosomal NTS region of this strain, in reference to the sequence of control strains isolated from fixed cutaneous sporotrichosis. After inoculated into immunosuppressed mice, this strain caused more extensive system involvement and showed stronger virulence than the control strain isolated from a fixed cutaneous sporotrichosis. Our study thus suggests that different clinical manifestation of sporotrichosis may be associated with variation in genotype and virulence of the strain, independent of effects due to the immune status of the host.

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Acknowledgments

This study is funded by National Natural Science Foundation of China.

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Authors and Affiliations

  1. Department of Dermatology, The 1st Affiliated Hospital of Dalian Medical University, No. 222, Zhongshan Road, Dalian, 116011, China

    Zhenying Zhang, Xiaoming Liu & Jingrong Lin

  2. Dalian Hospital of Dermatology, Dalian, China

    Xuelian Lv

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  1. Zhenying Zhang
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  2. Xiaoming Liu
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  3. Xuelian Lv
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  4. Jingrong Lin
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Correspondence to Xiaoming Liu.

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Zhang, Z., Liu, X., Lv, X. et al. Variation in Genotype and Higher Virulence of a Strain of Sporothrix schenckii Causing Disseminated Cutaneous Sporotrichosis. Mycopathologia 172, 439–446 (2011). https://doi.org/10.1007/s11046-011-9441-7

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  • DOI: https://doi.org/10.1007/s11046-011-9441-7

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