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Adhesive Properties and Hydrolytic Enzymes of Oral Candida albicans Strains

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Abstract

Several virulence factors in Candida albicans strains such as production of hydrolytic enzymes and biofilm formation on surfaces and cells can contribute to their pathogenicity. For this, control of this opportunistic yeast is one of the factors reducing the nosocomial infection. The aim of this study was to investigate biofilm formation on polystyrene and polymethylmethacrylate and the production of hydrolytic enzymes in Candida albicans strains isolated from the oral cavity of patients suffering from denture stomatitis. All strains were identified by macroscopic, microscopic analysis and the ID 32 C system. Our results showed that 50% of the total strains produced phospholipase. Furthermore, protease activity was detected in seven (35%) strains. All Candida albicans strains were beta haemolytic. All C. albicans strains adhered to polystyrene 96-well microtiter plate at different degrees, and the metabolic activity of C. albicans biofilm formed on polymethylmethacrylate did not differ between tested strains. The atomic force micrographs demonstrated that biofilm of Candida albicans strains was organized in small colonies with budding cells.

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Acknowledgments

We gratefully acknowledge Prof. Stefania Zanetti (University of Sassari, Italy) for standard Candida albicans strains and Mr. Moez Abroud from the Centre de Recherche Technologique de Borj Cédria (Tunisia), for technical assistance with the AFM technique.

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Authors and Affiliations

  1. Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Département de Microbiologie, Faculté de Pharmacie, Monastir, Tunisie

    Emira Noumi, Mejdi Snoussi & Amina Bakhrouf

  2. Departamento de Microbiologia y Ecología, Facultad de Farmacia, Universidad de Valencia, Burjassot, Valencia, Spain

    Emira Noumi

  3. Laboratoire de Microbiologie, Hôpital Ebn El Jazzar de Kairouan, Kairouan, Tunisie

    Kacem Mahdouani

  4. Departamento de Microbiologia y Ecología, Facultad de Farmacia, Universidad de Valencia, Burjassot, Valencia, Spain

    Lucas del Castillo, Eulogio Valentin & Rafael Sentandreu

  5. Service de Médecine et Chirurgie Buccales, Clinique Odontologique de Monastir, Monastir, Tunisie

    Hajer Hentati

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  1. Emira Noumi
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Correspondence to Emira Noumi.

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Noumi, E., Snoussi, M., Hentati, H. et al. Adhesive Properties and Hydrolytic Enzymes of Oral Candida albicans Strains. Mycopathologia 169, 269–278 (2010). https://doi.org/10.1007/s11046-009-9259-8

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  • DOI: https://doi.org/10.1007/s11046-009-9259-8

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