Skip to main content
SpringerLink
Log in

Inhibition of Candida albicans Biofilm Formation by Antimycotics Released from Modified Polydimethyl Siloxane

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Unlike various disinfectants, antifungals have not been commonly incorporated so far in medical devices, such as catheters or prostheses, to prevent biofilm formation by Candida spp. In the present study, five antimycotics were added to polydimethyl siloxane (PDMS) disks via admixture (nystatin) or impregnation (trimethylsilyl-nystatin (TMS-nystatin), miconazole, tea tree oil (TTO), zinc pyrithione). Nystatin-medicated PDMS disks exhibited a concentration-dependent inhibitory effect on biofilm formation in a microtiter plate (MTP) but not in a Modified Robbins Device (MRD). This observation, together with HPLC data and agar diffusion tests, indicates that a small fraction of free nystatin is released, which kills Candida albicans cells in the limited volume of a MTP well. In contrast, biofilm inhibition amounted to more than one log unit in the MRD on disks impregnated with miconazole, TTO, and zinc pyrithione. It is hypothesized that the reduction in biofilm formation by these compounds in a flow system occurs through a contact-dependent effect.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Kojic EM, Darouiche RO. Candida infections of medical devices. Clin Microbiol Rev. 2004;17(2):255–67.

    Article  PubMed  Google Scholar 

  2. Bauters TGM, Moerman M, Vermeersch H, Nelis HJ. Colonization of voice prostheses by albicans and non-albicans Candida species. Laryngoscope. 2002;112:708–12.

    Article  PubMed  Google Scholar 

  3. Ell SR. Candida-‘the cancer of silastic’. J Laryngol Otol. 1996;110:240–2.

    Article  CAS  PubMed  Google Scholar 

  4. Arweiler-Harbeck D, Sanders A, Held M, Jerman M, Ehrich H, Jahnke K. Does metal coating improve the durability of PDMS voice prostheses? Acta Otolaryngol. 2001;121:643–6.

    Article  CAS  PubMed  Google Scholar 

  5. Dijk F, Westerhof M, Busscher HJ, van Luyn MJA, van der Mei HC. In vitro formation of oropharyngeal biofilms on PDMS rubber treated with a palladium/tin salt mixture. J Biomed Mater Res. 2000;51:408–12.

    Article  CAS  PubMed  Google Scholar 

  6. Hetrick EM, Schoenfisch MH. Reducing implant-related infections: active release strategies. Chem Soc Rev. 2006;35:780–9.

    Article  CAS  PubMed  Google Scholar 

  7. Wu P, Grainger DW. Drug/device combinations for local drug therapies and infection prophylaxis. Biomaterials. 2006;27:2450–67.

    Article  CAS  PubMed  Google Scholar 

  8. Dowling DP, Betts AJ, Pope C, McConnell ML, Eloy R, Arnaud MN. Anti-bacterial silver coatings exhibiting enhanced activity through the addition of platinum. Surf Coat Technol. 2003;163–164:637–40.

    Article  Google Scholar 

  9. Gatter N, Kohnen W, Jansen B. In vitro efficacy of a hydrophilic central venous catheter loaded with silver to prevent microbial colonization. Zentralbl Bakteriol. 1998;287(1–2):157–69.

    CAS  PubMed  Google Scholar 

  10. Kwok CS, Wan C, Hendricks S, Bryers JD, Horbett TA, Ratner BD. Design of infection-resistant antibiotic-releasing polymers. I. Fabrication and formulation. J Control Release. 1999;62:289–99.

    Article  CAS  PubMed  Google Scholar 

  11. Kwok CS, Wan C, Horbett TA, Ratner BD. Design of infection-resistant antibiotic-releasing polymers. II. Controlled release of antibiotics through a plasma-deposited thin film barrier. J Control Release. 1999;62:301–11.

    Article  CAS  PubMed  Google Scholar 

  12. Price JS, Tencer AF, Arm DM, Bohach GA. Controlled release of antibiotics from coated orthopedic implants. J Biomed Mater Res. 1996;30:281–6.

    Article  CAS  PubMed  Google Scholar 

  13. Schierholz JM. Physico-chemical properties of a rifampicin-releasing polydimethylsiloxane shunt. Biomaterials. 1997;18:635–41.

    Article  CAS  PubMed  Google Scholar 

  14. Schierholz JM, Steinhauser H, Rump AFE, Berkels R, Pulverer G. Controlled release of antibiotics from biomedical polyurethanes: morphological and structural features. Biomaterials. 1997;18:839–44.

    Article  CAS  PubMed  Google Scholar 

  15. Stigter M, Bezemer J, de Groot K, Layrolle P. Incorporation of different antibiotics into carbonated hydroxyapatite coatings on titanium implants, release and antibiotic efficacy. J Control Release. 2004;99:127–37.

    Article  CAS  PubMed  Google Scholar 

  16. Von Eiff C, Kohnen W, Becker K, Jansen B. Modern strategies in the prevention of implant-associated infections. Int J Artif Organs. 2005;28(11):1146–56.

    Google Scholar 

  17. Kingston D, Seal DV, Hill ID. Self-disinfecting plastics for intravenous catheters and prosthetic inserts. J Hyg (Lond). 1986;96(2):185–98.

    CAS  Google Scholar 

  18. Darouiche RO, Mansouri MD, Kojic EM. Antifungal activity of antimicrobial-impregnated devices. Clin Microbiol Infect. 2006;12:397–9.

    Article  CAS  PubMed  Google Scholar 

  19. Maki DG, Stolz SM, Wheeler S, Mermel LA. Prevention of central venous catheter-related bloodstream infection by use of an antiseptic-impregnated catheter: a randomized, controlled trial. Ann Intern Med. 1997;127:257–66.

    CAS  PubMed  Google Scholar 

  20. Pigno MA, Goldschmidt MC, Lemon JC. The efficacy of antifungal agents incorporated into a facial prosthetic PDMS elastomer. J Prosthet Dent. 1994;71:295–300.

    Article  CAS  PubMed  Google Scholar 

  21. Groll AH, Mickiene D, Werner K, Piscitelli SC, Walsh TJ. High-performance liquid chromatographic determination of liposomal nystatin in plasma and tissues for pharmacokinetic and tissue distribution studies. J Chromatogr B. 1999;735:51–62.

    Article  CAS  Google Scholar 

  22. Groll AH, Mickiene D, Werner K, Petraitiene R, Petraitis V, Calendario M, et al. Compartimental pharmacokinetics and tissue distribution of multilamellar liposomal nystatin in rabbits. Antimicrob Agents Chemother. 2000;44:950–7.

    Article  CAS  PubMed  Google Scholar 

  23. Honraet K, Nelis HJ. Use of the Modified Robbins Device, fluorescent staining to screen plant extracts for the inhibition of S. mutans biofilm formation. J Microbiol Methods. 2006;64:217–24.

    Article  CAS  PubMed  Google Scholar 

  24. Coenye T, De Prijck K, De Wever B, Nelis HJ. Use of the Modified Robbins Device to study the in vitro biofilm removal efficacy of NitrAdine™, a novel disinfecting formula for the maintenance of oral medical devices. J Appl Microbiol. 2008;105(3):733–40.

    Article  CAS  PubMed  Google Scholar 

  25. Leunisse C, van Weissenbruch R, Busscher HJ, van der Mei HC, Albers FWJ. The artificial throat: a new method for standardization of in vitro experiments with tracheo-oesophageal voice prostheses. Acta Otolaryngol. 1999;119:604–8.

    CAS  PubMed  Google Scholar 

  26. De Prijck K, Nelis H, Coenye T. Efficacy of silver-releasing rubber for the prevention of Pseudomonas aeruginosa biofilm formation in water. Biofouling. 2007;23:405–11.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

Funding was provided by the Belgian Federation against Cancer and FWO-Vlaanderen. The authors are indebted to Imke Hindryckx and Carole Eyi Mba for their assistance in the work. The authors are also grateful to Philomain Wauters and Mario Podevijn from the Technical Workshop of the Faculty of Science for the construction of an optimized and miniaturized version of the MRD. We are also thankful to Prof. Hubert Vermeersch from the Head and Neck Surgery Department of the Ghent University Hospital.

Author information

Authors and Affiliations

  1. Laboratory of Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, Ghent, 9000, Belgium

    Kristof De Prijck, Kris Honraet, Steven Christiaen, Tom Coenye & Hans J. Nelis

  2. Laboratory of Polymer Chemistry and Biomaterials, Ghent University, Krijgslaan 281 S4bis, Ghent, 9000, Belgium

    Nele De Smet & Etienne Schacht

Authors
  1. Kristof De Prijck
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nele De Smet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kris Honraet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Steven Christiaen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tom Coenye
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Etienne Schacht
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hans J. Nelis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hans J. Nelis.

Additional information

Kristof De Prijck and Nele De Smet have contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

De Prijck, K., De Smet, N., Honraet, K. et al. Inhibition of Candida albicans Biofilm Formation by Antimycotics Released from Modified Polydimethyl Siloxane. Mycopathologia 169, 167–174 (2010). https://doi.org/10.1007/s11046-009-9242-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-009-9242-4

Keywords

Search

Navigation