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Comparative Tolerances of Various Beauveria bassiana Isolates to UV-B Irradiation with a Description of a Modeling Method to Assess Lethal Dose

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Abstract

The tolerances of 20 Beauveria bassiana isolates derived from host insects worldwide to UV-B irradiation were assessed quantitatively in multi-dose bioassays. Conidial suspensions of the isolates smeared on glass slides were exposed to the gradient UV-B doses of 0.1–1.6 J cm−2 (D), which generated from 0.75 to 10.17 min irradiation of weighted 312-nm wavelength at 2.0–2.61 mW cm−2. Irradiated conidia were then incubated for 24 h at 25°C under saturated humidity. The ratio of germination at each dose over that in the blank control was defined as survival index (I s). For all isolates, the I s − D observations fit well with the survival model I s = 1/[1 + exp(a + bD)] (0.94 ≤ r 2 ≤ 0.99) generated widely spanned lethal doses of 0.154–0.928, 0.240–1.139, and 0.383–1.493 J cm−2 for their losses of 50%, 75%, and 95% viabilities, respectively. These were far below the solar UV-B dose of 2.439 J cm−2 measured in a sunny day during the summer. The large variation of UV-B tolerance among the isolates indicates a necessity to select UV-tolerant candidates for formulations applied to insect control during summer. The highly efficient bioassay method was developed to measure accurately the UV-B tolerances of fungal biocontrol agents as lethal doses.

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Acknowledgments

We thank Humber RA (RW Holley Center for Agriculture and Health, Ithaca, NY, USA) for providing ARSEF fungal isolates. Funding of this study was provided jointly by the Ministry of Science and Technology of China (2009CB118904 and 2007DFA3100), and Zhejiang R&D Program (2007C12035 and 2008C12057).

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  1. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, People’s Republic of China

    Bao-Fu Huang & Ming-Guang Feng

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  1. Bao-Fu Huang
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  2. Ming-Guang Feng
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Correspondence to Ming-Guang Feng.

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Huang, BF., Feng, MG. Comparative Tolerances of Various Beauveria bassiana Isolates to UV-B Irradiation with a Description of a Modeling Method to Assess Lethal Dose. Mycopathologia 168, 145–152 (2009). https://doi.org/10.1007/s11046-009-9207-7

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  • DOI: https://doi.org/10.1007/s11046-009-9207-7

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