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An Oxidized Ergosterol from Pleurotus cystidiosus Active Against Anthracnose Causing Colletotrichum gloeosporioides

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Abstract

This study was undertaken to study the antifungal activity of Pleurotus cystidiosus against Colletotrichum gloeosporioides. This was achieved by fractionating the mushroom, P. cystidiosus initially to acetone (A), dichloromethane (D), and hexane (H) and studying the antifungal activity using the standard poisoned food technique. All the test solutions used were in the concentration of 20,000 ppm. The percentage inhibition of extracts A, D, and H was 12, 7, and 0.4%, respectively. Antifungal assay guided fractionation of the most active extract A resulted in four fractions; A1, A2, A3, and A4 having 12, 22, 0, and 17% percentage inhibitions, respectively. Fractions A2 and A4 were selected for further purifications. Normal phase column chromatography of A2 gave A2-1, A2-2, A2-3, and A2-4, with percentage inhibitions 7, 5, 26, and 13%, respectively. The fraction with the highest inhibitory activity (A2-3) was further separated using the Chromatotron and a single compound (A2-3-13) with 41% inhibition was isolated. Structure elucidation of this compound using 1D and 2D NMR spectroscopy proved this compound to be 3β, 5α, 6β-trihydroxyergosta-7,22-diene.

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Acknowledgments

We acknowledge the financial support form NSF grant RG/2004/C/2 (funds for consumables and research assistant) and for TWAS grant 05-328 RG/CHE/AS (2006) for purchasing the Chromatotron. We also acknowledge the technical support given by Ms. Nilakshi Silva, Dept. of Plant Science, University of Colombo in conducting the bioassays.

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Authors and Affiliations

  1. Department of Chemistry, University of Colombo, Colombo, Sri Lanka

    Inoka P. Menikpurage & D. T. U. Abeytunga

  2. Department of Chemistry, University of Arizona, Tucson, AZ, 85721, USA

    Neil E. Jacobsen

  3. Department of Plant Sciences, University of Colombo, Colombo, Sri Lanka

    R. L. C. Wijesundara

Authors
  1. Inoka P. Menikpurage
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  2. D. T. U. Abeytunga
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  3. Neil E. Jacobsen
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  4. R. L. C. Wijesundara
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Correspondence to D. T. U. Abeytunga.

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Menikpurage, I.P., Abeytunga, D.T.U., Jacobsen, N.E. et al. An Oxidized Ergosterol from Pleurotus cystidiosus Active Against Anthracnose Causing Colletotrichum gloeosporioides . Mycopathologia 167, 155–162 (2009). https://doi.org/10.1007/s11046-008-9158-4

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  • DOI: https://doi.org/10.1007/s11046-008-9158-4

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