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Species Distribution and Antifungal Susceptibility Profile of Oral Candida Isolates from HIV-infected Patients in the Antiretroviral Therapy Era

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Abstract

In this study, we investigated the yeasts colonization of genus Candida, including C. dubliniensis, isolated of HIV-infected patients oral cavities and we accessed in vitro susceptibility pattern of the Candida isolates to four antifungal agents. Out of 99 patients investigated, 62 (62.6%) were colonized with yeasts. C. albicans was the prevailing species (50%). C. dubliniensis isolates were not recovered in our study. We verified that 8.1% of the yeasts isolated were resistant to fluconazole, 8.1% to itraconazole and 3.2% to voriconazole. The isolates demonstrated very low voriconazole MICs, in which 79% (49/62) presented values of 0.015 μg/ml. All Candida isolates were susceptible to amphotericin B. The results reported here showed that although C. albicans continues to be present in one-half of oral Candida carriage of HIV-infected patients, Candida non-albicans species are increasing among these patients. Besides, the findings of resistant isolates endorse the role of antifungal susceptibility testing whenever antifungal treatment with azoles is planned.

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Abbreviations

DMSO:

Dimetyl sulfoxide

HDT:

Hospital de Doenças Tropicais

HIV:

Human Immunodeficiency Virus

MICs:

Minimal Inhibitory Concentrations

MOPS:

Morpholinepropanesulfonic acid

NCCLS:

National Committee for Clinical Laboratory Standards

OPC:

Oropharyngeal candidiasis

SDA:

Sabouraud Dextrose Agar

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Authors and Affiliations

  1. Instituto de Patologia Tropical e Saúde Pública da, Universidade Federal de Goiás, Goiás, Brazil

    Carolina Rodrigues Costa, Janine Aquino de Lemos, Xisto Sena Passos, Crystiane Rodrigues de Araújo, Lúcia Kioko Hasimoto e Souza & Maria do Rosário Rodrigues Silva

  2. Hospital de Doenças Tropicais de Goiás, Goiás, Brazil

    Ana Joaquina Cohen

  3. Maria do Rosário Rodrigues Silva, Rua 15, no 108, apto. 700, Setor Oeste, 74140-090, Goiania, GO, Brasil

    Maria do Rosário Rodrigues Silva

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  1. Carolina Rodrigues Costa
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  2. Janine Aquino de Lemos
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Correspondence to Maria do Rosário Rodrigues Silva.

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Costa, C.R., de Lemos, J.A., Passos, X.S. et al. Species Distribution and Antifungal Susceptibility Profile of Oral Candida Isolates from HIV-infected Patients in the Antiretroviral Therapy Era. Mycopathologia 162, 45–50 (2006). https://doi.org/10.1007/s11046-006-0032-y

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