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Detection of IgG and IgM in Sera from Canines with Blastomycosis using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens

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Abstract

The objective of this study was to compare the efficacy of eight Blastomyces dermatitidis yeast phase lysate antigens (T-58: dog, Tennessee; T-27: polar bear, Tennessee; ERC-2: dog, Wisconsin; B5894: human, Minnesota; SOIL: soil, Canada; B5896: human, Minnesota; 48089: human, Zaire; 48938: bat, India) in the detection of the immunoglobulins IgG and IgM in serum specimens from canines with blastomycosis. An indirect enzyme-linked immunosorbent assay (ELISA, peroxidase system) was used to analyze sera collected during four different intervals post-infection. The yeast lysate antigen 48938 was a reactive antigen for the detection of both IgG (mean absorbance value range: 1.198–2.934) and IgM (mean absorbance value range: 0.505–0.845). For the same sera, antigen T-27 was also effective in the detection of IgG (mean absorbance value range: 0.904–3.356) and antigen 48089 was useful for the detection of IgM (mean absorbance value range: 0.377–0.554). The yeast lysate antigen B5894 proved to be a poor antigen for the detection of both IgG and IgM (mean absorbance value ranges: 0.310–0.744 for IgG, 0.025–0.069 for IgM). Inherent variations in yeast lysate antigens such as these may be utilized to develop improved immunoassay procedures for the specific detection of IgG or IgM in cases of blastomycosis.

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References

  1. R Bradsher (1992) ArticleTitleBlastomycosis Clin Infect Dis 14 S82–S90 Occurrence Handle1314106

    PubMed  Google Scholar 

  2. P Murphy (1989) ArticleTitleBlastomycosis J Am Med 261 3159–3161 Occurrence Handle1:STN:280:DyaL1M3js1Gkug%3D%3D Occurrence Handle10.1001/jama.261.21.3159

    Article  CAS  Google Scholar 

  3. WA Causey GD Campbell (1992) Clinical aspects of blastomycosis Y Al-Doory AF DiSalvo (Eds) Blastomycosis Plenum Medical Book Co New York 165–188

    Google Scholar 

  4. RC Axtell GM Scalarone (2002) ArticleTitleSerological differences in three Blastomyces dermatitidis strains Mycoses 45 437–442 Occurrence Handle12472717 Occurrence Handle1:STN:280:DC%2BD38jhtFyisg%3D%3D

    PubMed  CAS  Google Scholar 

  5. SM Johnson GM Scalarone (1988) ArticleTitlePreparation and ELISA evaluation of Blastomyces dermatitidis yeast phase lysate antigens Diagn Microbiol Infect Dis 11 81–86 Occurrence Handle3229098 Occurrence Handle1:STN:280:DyaL1M7lt1Wqsw%3D%3D Occurrence Handle10.1016/0732-8893(88)90076-4

    Article  PubMed  CAS  Google Scholar 

  6. S Turner L Kaufman (1986) ArticleTitleImmunodiagnosis of blastomycosis Semin Respir Infec 1 22–28 Occurrence Handle1:STN:280:DyaL1c%2FmvFygsA%3D%3D

    CAS  Google Scholar 

  7. A Wakamoto BM Fryer MA Fisher et al. (1997) ArticleTitleDetection of antibodies and delayed dermal hypersensitivity with different lots of Blastomyces dermatitidis yeast lysate antigen: stability and specificity evaluations Mycoses 40 303–308 Occurrence Handle9476514 Occurrence Handle1:STN:280:DyaK1c7js12qsw%3D%3D Occurrence Handle10.1111/j.1439-0507.1997.tb00236.x

    Article  PubMed  CAS  Google Scholar 

  8. BW Seawell AM Legendre GM Scalarone (1990) ArticleTitleEnzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens Mycoses 33 483–489 Occurrence Handle2093845 Occurrence Handle1:STN:280:DyaK3M3jvV2jsQ%3D%3D

    PubMed  CAS  Google Scholar 

  9. RC Axtell GM Scalarone (2001) ArticleTitleSerological differences in two Blastomyces dermatitidis isolates from different geographical regions of North America Mycopathologia 153 141–144 Occurrence Handle10.1023/A:1014599115064

    Article  Google Scholar 

  10. EM Chester RC Axtell GM Scalarone (2003) ArticleTitle Blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis Mycopathologia 156 289–294 Occurrence Handle14682453 Occurrence Handle1:STN:280:DC%2BD3sngtVGlsw%3D%3D Occurrence Handle10.1023/B:MYCO.0000003575.04692.2f

    Article  PubMed  CAS  Google Scholar 

  11. JH Green WK Harrell JE Johnson et al. (1980) ArticleTitleIsolation of an antigen from Blastomyces dermatitidis that is specific for the diagnosis of blastomycosis Curr Microbiol 4 293–296

    Google Scholar 

  12. BJ Klein JN Kuritsky WA Chappel et al. (1986) ArticleTitleComparison of the enzyme immunoassay, immunodiffusion, and complement fixation tests in detecting antibody in human sera to the A antigen of Blastomyces dermatitidis Am Rev Respir Dis 133 144–148 Occurrence Handle3079974 Occurrence Handle1:STN:280:DyaL287gtFCrtA%3D%3D

    PubMed  CAS  Google Scholar 

  13. SH Turner L Kaufman M Jalbert (1986) ArticleTitleDiagnostic assessment of an enzyme-linked immunosorbent assay for human and canine blastomycosis J Clin Microbiol 23 294–297 Occurrence Handle2422200 Occurrence Handle1:STN:280:DyaL287ps1Kjug%3D%3D

    PubMed  CAS  Google Scholar 

  14. BS Klein JM Vergeront L Kaufman et al. (1987) ArticleTitleSerological tests for blastomycosis: assessments during a large point-source outbreak in Wisconsin J Infect Dis 155 262–268 Occurrence Handle3100658 Occurrence Handle1:STN:280:DyaL2s7htVynsg%3D%3D

    PubMed  CAS  Google Scholar 

  15. Kaufman, L. Immunodiagnosis of blastomycosis. In: Al-Doory Y, DiSalvo AF, eds. Blastomycosis, New York: Plenum Medical Book Co., 1992: 123–132

  16. RO Abuodeh EM Chester GM Scalarone (2004) ArticleTitleComparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources Mycoses 47 143–149 Occurrence Handle15078431 Occurrence Handle1:STN:280:DC%2BD2c7otlGhtw%3D%3D Occurrence Handle10.1111/j.1439-0507.2004.00972.x

    Article  PubMed  CAS  Google Scholar 

  17. BS Klein RA Squires JK Lloyd DR Ruge AM Legendre (2000) ArticleTitleCanine antibody response to Blastomyces dermatitidis WI-1 antigen Am J Vet Res 61 554–558 Occurrence Handle10803652 Occurrence Handle1:CAS:528:DC%2BD3cXjsFCgu74%3D Occurrence Handle10.2460/ajvr.2000.61.554

    Article  PubMed  CAS  Google Scholar 

  18. HB Levine GM Scalarone SD Chaparas (1977) ArticleTitlePreparation of fungal antigens and vaccines: studies on Coccidioides immitis and Histoplasma capsulatum Contrib Microbiol Immunol 3 106–125

    Google Scholar 

  19. GM Scalarone HB Levine SD Chaparas (1978) ArticleTitleDelayed hypersensitivity responses of experimental animals to histoplasmin from the yeast and mycelial phases of Histoplasma capsulatum Infect Immun 21 705–713 Occurrence Handle711329 Occurrence Handle1:STN:280:DyaE1M%2Fkslaktw%3D%3D

    PubMed  CAS  Google Scholar 

  20. HB Levine GM Scalarone GD Campbell PC Kelly SD Chaparas (1979) ArticleTitleHistoplasmin-CYL, a yeast phase reagent in skin test studies in humans Am Rev Respir Dis 119 629–636 Occurrence Handle443633 Occurrence Handle1:STN:280:DyaE1M7osFOrtA%3D%3D

    PubMed  CAS  Google Scholar 

  21. SF Hurst L Kaufman (1992) ArticleTitleWestern immunoblot analysis and serologic characterization of Blastomyces dermatitidis yeast form extracellular antigens J Clin Microbiol 30 3043–3049 Occurrence Handle1452683 Occurrence Handle1:CAS:528:DyaK3sXht1SmsbY%3D

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, Idaho State University, Box 8007, Pocatello, ID, 83209, USA

    Christine M. Sestero & Gene M. Scalarone

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  1. Christine M. Sestero
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  2. Gene M. Scalarone
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Correspondence to Christine M. Sestero.

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Sestero, C.M., Scalarone, G.M. Detection of IgG and IgM in Sera from Canines with Blastomycosis using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens. Mycopathologia 162, 33–37 (2006). https://doi.org/10.1007/s11046-006-0028-7

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  • DOI: https://doi.org/10.1007/s11046-006-0028-7

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