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Partial Purification and Characterization of a 37 kDa Extracellular Proteinase from Trichophyton vanbreuseghemii

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Abstract

An exocellular proteinase synthesized by the geophilic dermatophyte Trichophyton vanbreuseghemii has been purified and characterized. The fungus obtained from soil in Iran was cultivated in modified Czapek–Dox liquid medium containing 0.1% bacteriological peptone and 1% glucose as the nitrogen and carbon sources. Partial purification of the proteinase was accomplished by (NH4)2SO4 precipitation, followed by ion exchange chromatography. Analysis of the enzyme by SDS-PAGE revealed a single polypeptide chain with an apparent molecular mass of 37 kDa. Proteinase activity was optimum at pH 8, but remained high in the range of pH 7–11. Moreover, the partially purified enzyme presented a keratinolytic activity as evidenced by the keratin azure test. The inhibition profile and the good activity of the enzyme towards the synthetic substrate N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide suggested that it belonged to the chymotrypsin/subtilisin group of serine proteinases. The keratinolytic properties of T. vanbreuseghemii suggest that this fungus may be an alternative for the recycling of industrial keratinic wastes.

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Authors and Affiliations

  1. Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, 14155, Tehran, Iran

    Hossein Moallaei & Farideh Zaini

  2. Groupe d’Etude des Interactions Hôte-Parasite, UPRES-EA 3142, Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, 4, rue Larrey, 49033, Angers, France

    Gérald Larcher, Bertrand Beucher & Jean-Philippe Bouchara

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  1. Hossein Moallaei
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  2. Farideh Zaini
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  3. Gérald Larcher
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Correspondence to Farideh Zaini.

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Moallaei, H., Zaini, F., Larcher, G. et al. Partial Purification and Characterization of a 37 kDa Extracellular Proteinase from Trichophyton vanbreuseghemii . Mycopathologia 161, 369–375 (2006). https://doi.org/10.1007/s11046-006-0019-8

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  • DOI: https://doi.org/10.1007/s11046-006-0019-8

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