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Low temperature plasma suppresses proliferation, invasion, migration and survival of SK-BR-3 breast cancer cells

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Abstract

Background

Low temperature plasma (LTP) is a developing field in recent years to play important roles of sterilization, material modification and wound healing. Breast cancer is a common gynecological malignant tumor. Recent studies have shown that LTP is a promising selective anti-cancer treatment. The effect of LTP on breast cancer is still unclear. In this study, We treated breast cancer cell lines with low temperature plasma for different periods of time and analyzed the relevant differences.

Methods and results

SK-BR-3 cell nutrient solution was firstly treated by ACP for 0, 10, 20, 40, 80 and 120 s, which was next used to cultivateSK-BR-3cells for overnight.we found that LTP was able to suppress cell vitality, proliferation, invasion and migration of SK-BR-3 cells. Also, SK-BR-3 apoptosis was induced by LTP in a time-dependent manner.

Conclusion

These evidences suggest the negative effect of LTP on malignant development of SK-BR-3 cells, and LTP has the potential clinical application for breast cancer treatment.

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Availability of data and materials

All data are available from the corresponding author on reasonable request.

Abbreviations

LTP:

Low temperature plasma

ROS:

Reactive oxygen species

MAPK:

Mitogen-activated proteinkinase

Erks:

Extracellular signal-regulated kinases

NF-κB:

Nuclear factor kappa-B

PCNA:

Proliferating Cell Nuclear Antigen

BAX:

BCL2-associated X protein

BCL-2:

B-cell lymphoma-2

MMP2:

Matrix metalloproteinase 2

MMP9:

Matrix metalloproteinase 9

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Acknowledgements

None.

Funding

This research was supported by the fund from National Key R&D Program of China to Guohua Ni (grant number 2019YFC0119000), National Natural Science Foundation of China to Dong Wang, Guohua Ni and Qiying Shen (grant number 31800702, 11875295, 11535003 and 81902003), funds from Anhui Medical University to Dong Wang [grant number XJ201603 and 2017xkj003].

Author information

Author notes

  1. Xiang Li and Tao Sun contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, 230022, Hefei, Anhui, China

    Xiang Li

  2. Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, 230032, Hefei, Anhui, China

    Xiang Li

  3. Hefei Institutes of Physical Science, Institute of Plasma Physics, Chinese Academy of Sciences, P.O. Box 1126, 230031, Hefei, Anhui, China

    Tao Sun & GuoHua Ni

  4. University of Science and Technology of China, 230026, Hefei, Anhui, China

    Tao Sun & GuoHua Ni

  5. School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, China

    XinRu Zhang, Chao Hou & Dong Wang

  6. Department of Anesthesiology, Key Laboratory of Anesthesiology and Perioperative Medicine of Anhui Higher Education Institutes, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, China

    Qiying Shen

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  1. Xiang Li
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Contributions

XL: Conceptualization, Methodology, Writing Original draft preparation, Supervision. TS: Software, Data curation Writing-Reviewing. QS: Editing. CH: Data curation. XZ: Visualization. DW: Investigation. GN: Validation.

Corresponding authors

Correspondence to Qiying Shen, Dong Wang or GuoHua Ni.

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Li, X., Sun, T., Zhang, X. et al. Low temperature plasma suppresses proliferation, invasion, migration and survival of SK-BR-3 breast cancer cells. Mol Biol Rep 50, 2025–2031 (2023). https://doi.org/10.1007/s11033-022-08026-4

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