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Expression profiling of cancer-related long non-coding RNAs revealed upregulation and biomarker potential of HAR1B and JPX in colorectal cancer

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Abstract

Background

Aberrant expressions of long non-coding RNAs promote cancer development including colorectal cancer. Expression profiling of cancer-related lncRNAs may introduce new deregulated lncRNAs that might be recruited as novel platforms in diagnosis and therapy of CRC.

Methods and Results

In this study, we exploited the SBI Human LncProfiler qPCR Array to examine the expression pattern of 90 cancer-related lncRNAs in CRC samples. Among deregulated lncRNAs, HAR1B, JPX, and KRASP1- which were showed a significantly higher expression profile in aggressive CRC tumors- were selected for more validation. We found that HAR1B and JPX expression profiles may discriminate between adjacent, adenomatous colorectal polyps, and colorectal cancer samples. The area under the curve of near 0.7 and a sensitivity/specificity of more than 70.80%, respectively, claim a suitable cancer prognostic potential for these two lncRNAs, JPX and HAR1B. Further analysis revealed that HAR1B and JPX may contribute to CRC pathobiology through affecting the FOXO, ErbB, and Wnt/β-catenin signaling pathways.

Conclusions

Upregulated JPX and HAR1B lncRNAs may contribute to colorectal cancer pathobiology by affecting multiple cancer-related signaling pathways. They also potentially discriminate between CRC tumors, marginals, and adenomatous colorectal polyps.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Mina Khajehdehi, Mohammad Khalaj-Kondori & Mohammad Ali Hosseinpour Feizi

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  1. Mina Khajehdehi
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  2. Mohammad Khalaj-Kondori
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  3. Mohammad Ali Hosseinpour Feizi
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Contributions

Khalaj, as the project administrator, prepared the resources and has played a key role in the planning and methodology of the project with the cooperation of Khajehdehi. Writing- Original draft preparation, Investigation, and Software and Formal analysis has been done by Khajehdehi. Khalaj and Hosseinpour feizi contributed to the Review & Editing of the writing.

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Correspondence to Mohammad Khalaj-Kondori.

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The authors declare that there are no conflicts of interest.

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This study approved by the Ethics Committee of the University of Tabriz (IR.TABRIZU.REC.1398.027). It is also under the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Khajehdehi, M., Khalaj-Kondori, M. & Hosseinpour Feizi, M.A. Expression profiling of cancer-related long non-coding RNAs revealed upregulation and biomarker potential of HAR1B and JPX in colorectal cancer. Mol Biol Rep 49, 6075–6084 (2022). https://doi.org/10.1007/s11033-022-07396-z

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  • DOI: https://doi.org/10.1007/s11033-022-07396-z

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