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Isolation and characterization of microsatellites markers in Centaurium grandiflorum ssp. boissieri

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Abstract

Background

Estimating outcrossing/selfing rates and characterizing genetic diversity with microsatellite markers are crucial to understanding the evolution of plant mating systems.

Methods and results

We developed, optimized and characterized eight new primer pairs for Centaurium grandiflorum ssp. boissieri and transferred them to three subspecies of Centaurium quadrifolium. Two SSR loci were transferred from Sabatia campestris to the four Centaurium taxa. Polymorphisms, He, Ho and H–W deviations were estimated in two populations of C. grandiflorum ssp. boissieri and in seven individuals each of C. quadrifolium ssp. barrelieri, C. quadrifolium ssp. parviflorum and C. quadrifolium ssp. quadrifolium. A total of 80 individuals was used in these experiments. The number of polymorphic loci varied among species from one to ten. A total of 127 alleles was scored. The average number of alleles per locus was 12.7. He was higher than Ho in all sampled populations. Hardy–Weinberg equilibrium was found for some loci in different species.

Conclusions

This is the first report of microsatellites successfully amplified in the whole Centaurium genus. They will be valuable for estimating mating system parameters and genetic diversity and exploring their relationships with the wide variation in flower morphology in the genus, especially anther-stigma separation.

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Acknowledgements

We are thankful for the facilities provided by the CITIUS-2 centre, especially for the Greenhouse services and Herbarium of the University of Seville (SEV). VJL was supported by a postdoctoral fellowship from CONACYT (# 252042) and by a contract from the Ministerio de Economía, Industria y Competitividad (CGL2013-45037-P).

Funding

The research leading to these results received funding from two projects: PGC2018-099608-B-100 and, CGL2013-4503-P, funded by: FEDER / Ministerio de Ciencia e Innovación – Agencia Estatal de Investigación.

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Authors and Affiliations

  1. Laboratorio Nacional de Análisis y Síntesis Ecológica - Campus Costa Grande, Escuela Superior de Desarrollo Sustentable, Universidad Autónoma de Guerrero, Cátedras - CONACYT, Carretera Federal Acapulco - Zihuatanejo, Las Tunas, Tecpan de Galeana, Guerrero, México

    Vania Jiménez-Lobato

  2. Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain

    Vania Jiménez-Lobato, Marcial Escudero, Zoila Díaz Lifante, Cristina Andrés Camacho, Alejandra de Castro & Juan Arroyo

Authors
  1. Vania Jiménez-Lobato
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  2. Marcial Escudero
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  3. Zoila Díaz Lifante
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  4. Cristina Andrés Camacho
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  5. Alejandra de Castro
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  6. Juan Arroyo
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Contributions

JA and ME conceived the research topic. VJ-L, ZD-L, CAC and AC performed the field work and gathered the plant tissue. VJ-L, ME and AC performed the laboratory work. VJ-L performed the analyses. ME, JA and VJL wrote the manuscript. All authors contributed to the article and approved the submitted version.

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Correspondence to Vania Jiménez-Lobato.

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Jiménez-Lobato, V., Escudero, M., Lifante, Z.D. et al. Isolation and characterization of microsatellites markers in Centaurium grandiflorum ssp. boissieri. Mol Biol Rep 48, 8249–8253 (2021). https://doi.org/10.1007/s11033-021-06764-5

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