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Identification and characterization of multiple abiotic stress tolerance genes in wheat

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Abstract

Wheat is produced worldwide over six continents with the European Union, China, India, Russia, and the United States as major producer countries. The productivity was recorded 749 million tons by harvesting from 220-million-hectare land. It is the need of the hour to develop stress-tolerant wheat varieties to enhance the productivity by 60% to provide food security to 9.6 billion-world population by 2050. Although the genotypes have been identified for heat, drought and salt tolerance, their underlying mechanism for tolerance is poorly understood. The detailed understanding of the mechanism and identification of critical factors participating in multiple abiotic stress tolerance is essential. In the present study, the contrasting wheat genotypes were intensely characterized and assessed for the expression of different stress responsive genes under lab conditions. The expression analysis revealed that SHN1, DREB6, NHX2 and AVP1 were found to be highly induced under heat, salt and drought stresses in wheat. Thus, these genes can be used as signature genes to identify the multiple stress-tolerant varieties in the breeding program. The novel variants of these genes can be targeted through breeding or genetic engineering or genome editing strategies to develop multiple abiotic stress tolerant wheat varieties.

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Funding

This work is financially supported by the Indian Council of Agricultural Research, New Delhi, India, under the project entitled “ICAR Network Project on Functional Genomics and Genetic Modification in Crops (NPFGGM)” (Project No. 1006474).

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Authors and Affiliations

  1. ICAR-Indian Institute of Wheat and Barley Research (IIWBR), Karnal, Haryana, 132001, India

    Rakesh Kumar, Mamrutha Harohalli Masthigowda, Amandeep Kaur, Nabin Bhusal, Ankita Pandey, Satish Kumar, Chandranath Mishra, Gyanendra Singh & Gyanendra Pratap Singh

  2. Genetics and Plant Breeding, Faculty of Agriculture, Agricultural and Forestry University, Rampur, Chitwan, Nepal

    Nabin Bhusal

  3. Biosciences and Biotechnology, Banasthali Vidyapith, Jaipur, Rajasthan, 304022, India

    Ankita Pandey

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  1. Rakesh Kumar
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Contributions

MHM conceived and designed the research with RK. RK and AK conducted experiments. RK, AK, NB and AP collected experimental data. SK and CNM contributed analytical tools. RK and AK wrote the manuscript. MHM, GS and GPS edited the manuscript. All authors read and approved the manuscript for publication.

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Correspondence to Mamrutha Harohalli Masthigowda.

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Kumar, R., Masthigowda, M.H., Kaur, A. et al. Identification and characterization of multiple abiotic stress tolerance genes in wheat. Mol Biol Rep 47, 8629–8643 (2020). https://doi.org/10.1007/s11033-020-05906-5

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