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Hybrid of niosomes and bio-synthesized selenium nanoparticles as a novel approach in drug delivery for cancer treatment

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Abstract

The current study intends to investigate a novel drug delivery system (DDS) based on niosomes structure (NISM) and bovine serum albumin (BSA) which was formulated to BSA coated NISM (NISM-B). Also, selenium nanoparticles (SeNPs) have been prepared by BSA mediated biosynthesis. Finally, the NISM-B was hybridized with SeNPs and was formulated as NISM-B@SeNPs for drug delivery applications. Physicochemical properties of all samples were characterized by UV–Vis spectroscopy, FT-IR, DLS, FESEM, and EDX techniques. The cytotoxicity of all samples against A549 cell line was assessed by cell viability analysis and flow cytometry for apoptotic cells as well as RT-PCR for the expression of MDR-1, Bax, and Bcl-2 genes. Besides, in vivo biocompatibility was performed by LD50 assay to evaluate the acute toxicity. The proposed formulation has a regular spherical shape and approximately narrow size distribution with proper zeta-potential values; the proposed DDS revealed a good biocompatibility. The compound showed a significant cytotoxic effect against A549 cell line. Although the Bax/Bcl-2 expression ratio was significantly in NISM-B@SeNPs- treated cancer cells, the expression of MDR-1 was non-significantly lower in NISM-B@SeNPs-treated cancer cells. The obtained results suggest that the proposed DDS presents a promising approach for drug delivery, co-delivery and multifunctional biomedicine applications.

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Abbreviations

BSA :

Bovine serum albumin

NISM-B :

BSA coated NISM

cDNA :

Complementary DNA

DDS :

Drug delivery system

DLS :

Dynamic light scattering

FESEM :

Field emission scanning electron microscope

FT-IR :

Fourier-Transform Infrared

HAS :

Human serum albumin

LD 50 :

Median lethal dose

MDR :

Multidrug resistance

MLV :

Multilamellar vesicles

NISM :

Niosomes

KBr :

Potassium bromide

PDI :

Polydispersity index

PI :

Propidium iodide

RBC :

Red blood cells

RT :

Reverse-transcribed

RESs :

Reticulo-endothelial systems

SeNPs :

Selenium nanoparticles

UV–Vis :

Ultraviolet–Visible

ULV :

Unilamellar vesicles

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Acknowledgments

This study was supported financially by Zanjan University of Medical Sciences, Zanjan, Iran (Grant Number: A-12-1244-11 & Ethical Code: IR.ZUMS.REC.1398.438).

Author information

Authors and Affiliations

  1. Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran

    Mahmoud Gharbavi

  2. Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran

    Mahmoud Gharbavi

  3. Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran

    Mahmoud Gharbavi & Ali Sharafi

  4. Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

    Behrooz Johari, Navid Mousazadeh & Milad Parvinzad Leilan

  5. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Bahareh Rahimi

  6. Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

    Seyed Sadegh Eslami

Authors
  1. Mahmoud Gharbavi
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  2. Behrooz Johari
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  3. Navid Mousazadeh
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  6. Seyed Sadegh Eslami
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  7. Ali Sharafi
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Contributions

MG: Methodology, Conceptualization, Software, and Writing—original draft. BJ: Supervision, Project administration, Writing—review and editing, and Final approved. NM: Methodology. BR: Methodology. MPL: Methodology. SSE: Methodology. AS: Project administration and Writing—review and editing.

Corresponding authors

Correspondence to Behrooz Johari or Ali Sharafi.

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Conflict of interest

Behrooz Johari received the grant from Zanjan University of Medical Sciences. All the authors declare no financial or commercial conflict of interests that could negatively influence the study.

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There was no human participant and consent was not required.

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No human or animal was involved in this study.

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11033_2020_5704_MOESM1_ESM.tif

Fig. S1. (A, B) Rheological behavior of all samples. Non-Newtonian rheological behavior was observed for all samples. (C) Stability of all samples according to Z-Average (nm) as time dependent. Values with P< .05(*) and P< .01(**) were regarded statistically meaningful. Supplementary file1 (TIF 627 kb)

Supplementary file2 (DOCX 16 kb)

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Gharbavi, M., Johari, B., Mousazadeh, N. et al. Hybrid of niosomes and bio-synthesized selenium nanoparticles as a novel approach in drug delivery for cancer treatment. Mol Biol Rep 47, 6517–6529 (2020). https://doi.org/10.1007/s11033-020-05704-z

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  • DOI: https://doi.org/10.1007/s11033-020-05704-z

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