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Secondary metabolites from acridocarpus orientalis inhibits 4T1 cells and promotes mesenchymal stem cells (MSCs) proliferation

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Abstract

Among medicinal plants, Acridocarpus orientalis (AO) possesses a remarkable anti-cancer potential, possibly because of its anti-oxidant property. In this study, the leaf and stem extracts from AO were assessed to find the bioactive compound with selective anti-cancer properties. The MTT viability and live and dead assays revealed that around 80% and 98% of 4T1 cells survival were declined after 48 h incubation with leaf and stem extracts, respectively. The leaf extract increased stem cell proliferation by 20% whereas the stem extract inhibited around 22% of stem cells proliferation after 48 h treatment. The live and dead assay of MSCs confirmed that 40% of the MSCs died when treated with AO stem extract. On the other hand, there were no dead cells after two days of treatment with the leaf extract. Followed by the induction of cell cycle arrest in G0/G1-phase, the real-time PCR demonstrated apoptosis properties in 4T1 cells through overexpression of Bax and down-regulation of BCL2 genes. Interestingly, within the pure compounds isolated from AO leaf extract, Morin was responsible for the inhibition of 4T1 cells proliferation as well as MSCs expansion, predicting to play an essential role in the treatment of cancer. The promising in vitro anti-cancer and stem cell-inductive properties of morin isolated from AO extract may provide a great potential to produce selective herbal derived drugs.

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Acknowledgements

The authors acknowledge financial and technical support provided by University of Nizwa and Sultan Qaboos University.

Funding

This research was funded by The Research Council external grant number 169, 2017 and the GCC co-funding, grant number CL/SQU-GCC/17/03.

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Authors and Affiliations

  1. Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, P. O. Box: 33, Nizwa, PC, 616, Oman

    Fatemeh Jamshidi-adegani, Saeid Vakilian, Najeeb Ur Rehman, Mohammed Al-Broumi, Juhaina Al-kindi, Ahmed Al-Harrasi & Sulaiman Al-Hashmi

  2. Department of Mechanical and Industrial Engineering, Sultan Qaboos University, 123, Muscat, Oman

    Khurshid Alam

  3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Parisa Mozafarinahavandi

  4. Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, 2713, Doha, Qatar

    Anwarul Hasan

  5. Biomedical Research Center, Qatar University, 2713, Doha, Qatar

    Anwarul Hasan

  6. Department of Genetics, College of Medicine and Health Sciences, Sultan Qaboos University, PC 123, PO Box 35, Muscat, Oman

    Hamad Al-Riyami

  7. Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Nizwa, 616, Sultanate of Oman

    Javid Hussain

  8. Department of Engineering Science, Oxford University, Oxford, UK

    Solomon White

Authors
  1. Fatemeh Jamshidi-adegani
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  2. Saeid Vakilian
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  7. Parisa Mozafarinahavandi
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  11. Solomon White
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Contributions

Conceptualization, SV and SA-H; Formal analysis, MB; Funding acquisition, KA, AH and AAl-H; Methodology, FJ-a and JA-k (Cell culture techniques), NUR, MB, and JH (extraction and identification of compounds), SA-H and SV (animal handling and bone marrow extraction); Project administration, FJ-a and SA-H; Resources, PM, HA-R and AA-H; Supervision, AA-H and SA-H; Writing the original draft, SV; Writing – review & editing, KA, Solomon White, AA-H and SA-H.

Corresponding authors

Correspondence to Ahmed Al-Harrasi or Sulaiman Al-Hashmi.

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The authors declare no conflict of interest.

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Research involving human and animals rights

The animal experiments were done according to the guidelines of the National Committee of Bioethics and the institutional Animal Ethics Committee with Ethical approval No. VCGSR/AEC/05/2019. The mice were kept under standard requirements for housing, feeding, and breeding. The mice were sacrificed by cervical displacement to isolate the bone marrow-derived stem cells.

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Jamshidi-adegani, F., Vakilian, S., Rehman, N.U. et al. Secondary metabolites from acridocarpus orientalis inhibits 4T1 cells and promotes mesenchymal stem cells (MSCs) proliferation. Mol Biol Rep 47, 5421–5430 (2020). https://doi.org/10.1007/s11033-020-05632-y

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