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Identification and expression analysis of candidate genes associated with stem gall disease in Coriander (Coriandrum sativum L.) cultivars

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Abstract

Coriander (Coriandrum sativum L.) is a well-known spice and aromatic crop cultivated globally. Stem gall disease is one of the major constraints for its leaf and seed quality used for consumption and also affecting the yield. The identification of resistance genes and further characterization of such genes could help to understand the molecular basis of resistance and lay a solid ground for cloning of stem gall resistance genes in coriander. To evaluate the genetic expression of disease resistance-relevant genes in popularly grown coriander cultivars in India such as Pant Haritma, Hisar Sugandh, Hisar Surabhi, Hisar Anand, Rajendra Swathi, ACr-1, ACr-2, AgCr-1, CO-2 and CS-6 were used for LRR, GDSL, USP, ANK and PDR gene expression using Real Time PCR along with 18S housekeeping gene as internal control for the normalization. Result revealed the different expression pattern of genes among the cultivars tested. Highest expression was shown in cultivar AgCr-1 followed by Pant Haritma, Hisar Sugandh and ACr-1, and least expression in Hisar Anand, ACr-2, CO-2, Rajendra Swathi and CS-6. Domain analysis revealed the conserved domain relevance of the genes. This is the first report on stem gall resistance gene expression in coriander. The identified genes have a potential role in coriander and further utilize in crop improvement program. We hypothesize that contrasting cultivars can be a good source for candidate gene evaluation and further to use them as potential markers and used in hybridization program focus on incorporating and develop durable disease-resistance into the adapted cultivars of the region.

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Abbreviations

RT-qPCR:

Real-time quantitative PCR

LRR:

Leucine rich repeat

GDSL:

Gly-Asp-Ser-(Leu) motif

USP:

Universal stress protein

ANK:

Ankyrin repeat

PDR:

Probable disease resistance

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Acknowledgements

We thank Director, NRCSS for timely support and encouragement during the research work. The research is supported by the ICAR-NRC on Seed Spices, Ajmer, Rajasthan, India. Authors also acknowledge the support received by the UHS, Bagalkote, Karnataka for infrastructure facilities.

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Authors and Affiliations

  1. ICAR-National Research Centre on Seed Spices, Tabiji, Ajmer, Rajasthan, 305 206, India

    Sharda Choudhary & R. D. Meena

  2. Department of Biotechnology and Crop Improvement, K. R. C. College of Horticulture, Arabhavi, University of Horticultural Sciences, Bagalkote, Karnataka, 591 218, India

    Mahantesha B. N. Naika

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  1. Sharda Choudhary
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  3. R. D. Meena
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Correspondence to Sharda Choudhary.

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Choudhary, S., Naika, M.B.N. & Meena, R.D. Identification and expression analysis of candidate genes associated with stem gall disease in Coriander (Coriandrum sativum L.) cultivars. Mol Biol Rep 47, 5403–5409 (2020). https://doi.org/10.1007/s11033-020-05630-0

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