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A novel bacterial carboxylesterase identified in a metagenome derived-clone from Brazilian mangrove sediments

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Abstract

A functional screening of 1152 clones from a plasmid library constructed with DNA extracted from Brazilian mangrove sediments revealed 3 positive clones for ester-hydrolyzing enzymes, or about one lipolytic gene per 1.2 Mb DNA, which corroborates the idea that oil-contaminated mangroves are a good source of novel microbial lipases/esterases. The partial sequence of the clone LipG7 (1179 bp) showed 30.2% of predicted structure identity with a known esterase, confirming LipG7 as a new member of family VIII esterases. LigG7 shared 80% sequence identity with 1,4-butanediol diacrylate esterase from the Gammaprotebacteria Porticoccus hydrocarbonoclasticus, suggesting it belongs to the Porticoccaceae family. LipG7 was heterologously expressed in Escherichia coli Rosetta-Gami DE3; the purified recombinant enzyme exhibited a predicted molecular weight of 45.2 kDa and exceptional activity towards 4-nitrophenyl butyrate, compared with other recombinant esterases, highlighting its enormous potential for biological applications.

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Acknowledgements

The authors acknowledge the contributions made by the blinded reviewers and are grateful to Thomas Hitch (Institute of Medical Microbiology, Aachen, Germany) for the help with the enzyme classification analysis. The authors thank the National Council for Scientific and Technological Development (CNPq) and Foundation for Scientific and Technological Development of Ceará (FUNCAP) for the grant provided to LRBG (PRONEX-PR2-0101-00012.01.00/15) and for the scholarship provided to FJA. Collection of samples was registered by the pertinent environmental agency (A982E7D-SisGen).

Funding

The authors thank the National Council for Scientific and Technological Development (CNPq) and Foundation for Scientific and Technological Development of Ceará (FUNCAP) for the grant provided to LRBG (PRONEX-PR2-0101–00012.01.00/15) and for the scholarship provided to FJA.

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Authors and Affiliations

  1. Laboratório de Ecologia Microbiana e Biotecnologia (LEMBiotech), Departamento de Biologia, Universidade Federal do Ceará, Av. Humberto Monte 2977, Campus do Pici, Bloco 909, Fortaleza, CE, 60455-760, Brazil

    Francisco J. Araujo, Denise C. Hissa, Gabrielly O. Silva, André S. L. M. Antunes & Vânia M. M. Melo

  2. Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Av. da Abolição 3, Centro, Redenção, CE, 62790-000, Brazil

    Vanessa L. R. Nogueira

  3. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, CE, 60455-760, Brazil

    Luciana R. B. Gonçalves

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  1. Francisco J. Araujo
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  2. Denise C. Hissa
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  3. Gabrielly O. Silva
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  4. André S. L. M. Antunes
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  5. Vanessa L. R. Nogueira
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  6. Luciana R. B. Gonçalves
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  7. Vânia M. M. Melo
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Francisco J. Araujo, Gabrielly O. Silva and Vanessa L.R Nogueira. Luciana R. B. Gonçalves has contributed with funding acquisition and resources. Formal analysis and investigation were performed by Denise C. Hissa and Vânia M. M. Melo. The first draft of the manuscript was written by Denise C. Hissa and André S. L. M. Antunes. Review and editing of the manuscript was performed by Vânia M. M. Melo. All authors read and approved the final manuscript.

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Correspondence to Vânia M. M. Melo.

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Collection of samples was registered by the pertinent environmental agency (A982E7D-SisGen).

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Araujo, F.J., Hissa, D.C., Silva, G.O. et al. A novel bacterial carboxylesterase identified in a metagenome derived-clone from Brazilian mangrove sediments. Mol Biol Rep 47, 3919–3928 (2020). https://doi.org/10.1007/s11033-020-05484-6

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