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Analysis of ciliogenesis process in the bovine oviduct based on immunohistochemical classification

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Abstract

The oviductal epithelium is composed of ciliated and non-ciliated cells. The proportions of these cells change during the estrous cycle. However, the mechanism underlying this cyclic change in the cell proportions remains unclear. Our previous study indicated that ciliated cells are derived from non-ciliated cells. Here, we aimed to investigate the mechanism regulating the changes in the populations of ciliated and non-ciliated cells during the estrous cycle. To this end, we examined the numbers of cells that were positive for acetylated-α-tubulin (cilia marker), Ki67 (proliferation marker), PAX8 (non-ciliated cell marker), and FOXJ1 and MYB (ciliogenesis markers) in the epithelial cells at four different estrous stages (Stage I: days 1–4 after ovulation, Stage II: days 5–10, Stage III: days 11–17, and Stage IV: days 18–20) by immunohistochemistry. The oviductal epithelial cells expressed either FOXJ1 or PAX8. All the acetylated-α-tubulin+ cells were positive for FOXJ1, although there were a few acetylated-α-tubulin−/FOXJ1+ cells. MYB was expressed in both the FOXJ1+ and PAX8+ cells, but it was not expressed in the Ki67+ cells. The numbers of Ki67+ and MYB+ cells were the highest in Stage IV, while the numbers of FOXJ1+ and acetylated-α-tubulin+ cells were the highest in the following Stage I, suggesting that ciliogenesis is associated with the estrous cycle. Thus, based on immunological classification, the oviductal epithelium contains at least seven types of cells at different translational/transcriptional states, and their number is regulated by the estrous cycle. This cyclic event might provide an optimal environment for gamete transport, fertilization, and embryonic development.

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Funding

This work was supported by JSPS KAKENHI Grant Numbers 17H05041 (YY), and Grant-in-Aid for JSPS Fellows Grant Numbers 18J14047 (SI).

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Authors and Affiliations

  1. Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, 1-1-1, Tsushimanaka, Kita-Ku, Okayama, 700-8530, Japan

    Sayaka Ito, Yuki Yamamoto & Koji Kimura

  2. Japan Society for the Promotion of Science, Tokyo, Japan

    Sayaka Ito

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  1. Sayaka Ito
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  2. Yuki Yamamoto
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Correspondence to Koji Kimura.

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Ito, S., Yamamoto, Y. & Kimura, K. Analysis of ciliogenesis process in the bovine oviduct based on immunohistochemical classification. Mol Biol Rep 47, 1003–1012 (2020). https://doi.org/10.1007/s11033-019-05192-w

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