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Association between serum cell adhesion molecules with hs-CRP, uric acid and VEGF genetic polymorphisms in subjects with metabolic syndrome

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Abstract

Metabolic syndrome (MetS) is associated with a pro-inflammatory state and endothelial dysfunction that places subjects with MetS at a higher risk of atherosclerosis. Inflammatory biomarkers are raised in patients at risk of developing cardiovascular diseases. In the current study, we aimed to examine the possible association between MetS and serum soluble adhesion molecules, hs-CRP, uric acid, and the genetic variations related to vascular endothelial growth factor (VEGF) gene. In this cross-sectional study, participants were enrolled from the Mashhad stroke and heart atherosclerotic disorders (MASHAD) study. The International Diabetes Federation criteria were used to define the MetS. Cell adhesion molecules (CAM) and serum hs-CRP were measured by ELISA and PEG-enhanced immunoturbidimetry method, respectively. We used a logistic regression analysis to determine independent associations of CAMs with the VEGF polymorphisms and MetS. Two hundred and 59 participants with and without MetS were enrolled. Participants with MetS and DM had a significantly higher serum E-selectin level (p < 0.05). Participants with a high serum E-selectin level had higher levels of hs-CRP, FBG, TG, uric acid, BMI and lower levels of serum HDL-C (p < 0.05). Interestingly, individuals with MetS with a genetic variant of the VEGF gene (rs6921438) had higher level of serum ICAM-1 (p = 0.04). There were significant associations between serum E-selectin concentrations and the presence of MetS, and its risk factors. Moreover, we demonstrated that MetS subjects with the rs6921438 genetic variant had a higher serum level of ICAM-1 (p < 0.05).

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Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.

Abbreviations

IDF:

International diabetes federation

Hs-CRP:

High sensitivity C reactive protein

HDL:

High-density lipoprotein

LDL:

Low-density lipoprotein

TG:

Triglycerides

SNP:

Single nucleotide polymorphism

WC:

Waist circumference

DBP/SBP:

Diastolic/systolic blood pressure

FBG:

Fasting blood glucose

MUMS:

Mashhad University of Medical Science

BMI:

Body mass index

PCR:

Polymerase chain reaction

SD:

Standard deviation

IQR:

Interquartile range

CAD:

Coronary artery disease

HC:

Hip circumference

ICAM-1:

Intercellular adhesion molecule-1

VCAM-1:

Vascular adhesion molecule-1

CAM:

Cell adhesion molecule

VEGF:

Vascular endothelial growth factor

RFLP-PCR:

Polymerase chain reaction-restriction fragment length polymorphism

ARMS-PCR:

Polymerase chain reaction-amplification refractory mutation system

ELISA:

Enzyme-linked immunosorbent assay

IL:

Interleukin

TNF-α:

Tumor necrosis factor-alpha

G-CSF:

Granulocyte-colony stimulating factor

CVD:

Cardiovascular disease

CD62E:

CD62 antigen-like family member E

NF-KB:

Nuclear factor-kB

CKD:

Chronic kidney diseases

PI:

Phosphatidylinositol

MASHAD:

Mashhad stroke and heart atherosclerotic disorders

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Acknowledgements

We would like to thank the Mashhad University of Medical Sciences Research Council for their financial support.

Funding

This study was supported by a grant from the Research Council of the Mashhad University of Medical Sciences (Grant Nos. 921941 and 901033). It granted parts of the design, running or reporting of our study and the approval number from the constituted review board, the Ethics Committee of Mashhad University of Medical Sciences (Mums) is IR.MUMS.MEDICAL.REC.1386.250.

Author information

Authors and Affiliations

  1. Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran

    Hamideh Ghazizadeh & Amir Avan

  2. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, 99199-91766, Iran

    Hamideh Ghazizadeh, Majid Rezaei, Amir Avan, Alireza Pasdar, Shima Tavallaie & Majid Ghayour-Mobarhan

  3. Department of Biochemistry, Payame Noor University, Tehran, Iran

    Mohammad Fazilati

  4. Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

    Alireza Pasdar

  5. Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Elham Kazemi

  6. Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

    Seyed Mohammad Reza Seyedi

  7. Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

    Seyed Mohammad Reza Seyedi

  8. Division of Medical Education, Brighton & Sussex Medical School, Falmer, Brighton, Sussex, BN1 9PH, UK

    Gordon A. Ferns

  9. Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohsen Azimi-Nezhad

  10. UMR INSERM U 1122, IGE-PCV “Interactions Gène-Environnement en Physiopathologie CardioVasculaire”, Université de Lorraine, 54000, Nancy, France

    Mohsen Azimi-Nezhad

  11. School of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran

    Mohsen Azimi-Nezhad

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  1. Hamideh Ghazizadeh
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  2. Majid Rezaei
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  3. Amir Avan
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  10. Mohsen Azimi-Nezhad
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  11. Majid Ghayour-Mobarhan
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Contributions

We declare that We contributed significantly towards the research study i.e., (a) conception, design and/or analysis and interpretation of data and to (b) drafting the article or revising it critically for important intellectual content and on (c) final approval of the version to be published.

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Correspondence to Mohsen Azimi-Nezhad or Majid Ghayour-Mobarhan.

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Informed consent was obtained from all subjects using protocols approved by the Ethics Committee of the Mashhad University of Medical Sciences.

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Ghazizadeh, H., Rezaei, M., Avan, A. et al. Association between serum cell adhesion molecules with hs-CRP, uric acid and VEGF genetic polymorphisms in subjects with metabolic syndrome. Mol Biol Rep 47, 867–875 (2020). https://doi.org/10.1007/s11033-019-05081-2

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