Abstract
Colorectal cancer (CRC) is the third most common cancer in both men and women. Oncolytic viral-based therapy methods seem to be promising for CRC treatment. Vesicular stomatitis virus (VSV) is considered as a potent candidate in viral therapy for several tumors. VSV particles with mutated matrix (M) protein are capable of initiating cell death cascades while not being harmful to the immune system. In the current study, the effects of the VSV M-protein was investigated on the apoptosis of the colorectal cancer SW480 cell. Wild-type, M51R, and ΔM51 mutants VSV M-protein genes were cloned into the PCDNA3.1 vector and transfected into the SW480 cells. The results of the MTT assay, Western blotting, and Caspase 3, 8, and 9 measurement, illustrated that both wild and M51R mutant M-proteins can destroy the SW480 colorectal cancer cells. DAPI/TUNEL double-staining reconfirmed the apoptotic effects of the M-protein expression. The ΔM51 mutant M-protein is effective likewise M51R, somehow it can be considered as a safer substitution.
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Acknowledgements
The authors gratefully appreciate Mrs. Javid and Mr. Bazouri, research scientists at the Department of Microbiology, Golestan University of Medical Sciences (GoUMS) for their assistance in this work. This research was conducted at the laboratory of the Department of Microbiology as well as the Cell and Molecular Research Center, GoUMS and financially supported by the Deputy of Research and Technology, GoUMS, Gorgan, IR.
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ZG and MRK did the experiments, data analysis and text preparation; AT and AM did the project design, data analysis and discussion. AM was the PI of the project. All authors have seen, and approved the manuscript.
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Gray, Z., Tabarraei, A., Moradi, A. et al. M51R and Delta-M51 matrix protein of the vesicular stomatitis virus induce apoptosis in colorectal cancer cells. Mol Biol Rep 46, 3371–3379 (2019). https://doi.org/10.1007/s11033-019-04799-3
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DOI: https://doi.org/10.1007/s11033-019-04799-3