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Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice

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Abstract

Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases. N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders. However, the relationship between N-glycosylation pattern and depression is not well elucidated to date. This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model. We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group. Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype. Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile. N-glycan expression levels were commonly altered in the depressive-like model and correlated well with the behavioral data. Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression.

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Acknowledgements

The authors would like to express sincere thanks to the Drug Discovery Research Group at Nishimura Laboratory, Faculty of Advanced Life Sciences, Hokkaido University, Japan, for learning the glycomics technique. Thanks to Sohag University to fund the project, proposal ID: #271, appreciations to Dr. Michael G. Wolfe at Biointerfaces Institute, McMaster University, Hamilton, Ontario, Canada for the language edition and Prof. Mahmoud Salah El-Tarabany, head of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt for the statistical revision.

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Authors and Affiliations

  1. Department of Animal Behavior and Husbandry, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt

    Motamed Elsayed Mahmoud

  2. Department of Animal Behavior and Husbandry, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt

    Ibrahim F. Rehan & Eslam K. H. Ahmed

  3. Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, El-Zeraa Street, 114, Zagazig, 44511, Egypt

    Kh. El-Dawy Ahmed & Asmaa Elnagar

  4. Department of Physiology, Faculty of Medicine, Sohag University, Sohag, 82524, Egypt

    Amany Abdelrahman

  5. Department of Tissue Engineering and Applied Cell Sciences, University of Medical Sciences, Tehran, 1985711151, Iran

    Saeed Mohammadi

  6. Biointerfaces Institute, McMaster University, 1280 Main St W, Hamilton, ON, L8S 0A3, Canada

    Saeed Mohammadi

  7. Department of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

    Ahmed F. Abou-Elnaga

  8. Department of Animal Physiology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt

    Mohammed Youssef

  9. Department of Animal Hygiene, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt

    Hassan Mahmoud Diab

  10. Department of Animal Medicine, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt

    Doaa Salman

  11. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, El-Zeraa Street, 114, Zagazig, 44511, Egypt

    Hesham H Mohammed

  12. Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt

    Obeid Shanab

  13. Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt

    Rawia M. Ibrahim

  14. Genetics Department, Faculty of Agriculture, Assiut University, Assiut, 71516, Egypt

    Abd El-Latif Hesham

  15. Department of Zoology, Sri Avadh Raj Singh Smarak Degree College, Gonda, India

    Arti Gupta

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  1. Motamed Elsayed Mahmoud
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  2. Ibrahim F. Rehan
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  6. Ahmed F. Abou-Elnaga
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Contributions

MEM and IFR designed the survey protocol, conceived the study, supervised data collection procedures, and drafted the final version of the manuscript; KhEl-DA, AA, SM, EKHA and AFA-E wrote the statistical analysis, analysed the data and shared in experimental protocol; MY, HMD, DS, AE, HHM, AEl-LH, RMI, OS and AG have finalized the experimental design and revised the manuscript. All authors contributed to, edited, and approved the final manuscript as submitted.

Corresponding authors

Correspondence to Motamed Elsayed Mahmoud, Ibrahim F. Rehan or Abd El-Latif Hesham.

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The authors declare that they have no conflict of interests.

Ethical approval

The protocol was approved by the Ethics Committee for Animal Experimentation at Sohag University, Faculty of Medicine, Faculty Council No (282), Decree (7463), 14th September 2017.

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Mahmoud, M.E., Rehan, I.F., El-Dawy Ahmed, K. et al. Identification of serum N-glycoproteins as a biological correlate underlying chronic stress response in mice. Mol Biol Rep 46, 2733–2748 (2019). https://doi.org/10.1007/s11033-019-04717-7

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