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Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case–control study

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Abstract

Inflammatory cytokine, adipokine and adhesion molecules are known to play a key role in pathogenesis of diabetic kidney disease (DKD). In this study, our aim was to investigate the role of fetuin-A in relation with pro-inflammatory cytokines (IL-6, IL-18), adipokines (adiponectin, leptin), chemokine (MCP-1), and adhesion molecules (ICAM-1, VCAM-1) in control and DKD subjects. We recruited a total of 224 type 2 diabetic (T2D) subjects. The control subjects were T2D with a normal albumin excrete (albumin-to-creatinine ratio—ACR ≤ 30 mg/g creatinine) and estimated glomerular filtration rate (eGFR) ≥ 60 (ml/min/1.73 m2), while cases were T2D subjects with albumin excrete (ACR ≥ 30 mg/g creatinine) and eGFR ≤ 60 (ml/min/1.73 m2). FBS, HbA1c, lipid profile (TC, LDL, HDL, triglyceride), ALT, AST, GGT, serum creatinine, BMI, blood pressure was evaluated in all the study subjects. Randox evidence biochip analyzer was used for measuring inflammatory cytokines, adipokines, and adhesion molecules by chemiluminescent assay. Serum fetuin-A and IL-18 were measured by ELISA kits. Serum fetuin-A levels were significantly decreased in DKD cases compare to control group [456.8 (299.2–649.0) µg/ml versus 670.6 (573.0–726.1) µg/ml; p < 0.001)]. Serum fetuin-A levels correlates significantly with IL-6, IL-18, TNF-α, PAI-1, leptin, resistin and ACR (p < 0.001). This study concludes that serum fetuin-A and pro-inflammatory markers (IL-18, IL-6, IL-1α and TNF-α) might play an important role in the pathophysiology and inflammatory process of DKD.

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Authors and Affiliations

  1. Strategic Center for Diabetes Research, College of Medicine, King Saud University, P.O. Box 245, Riyadh, 11411, Kingdom of Saudi Arabia

    Shaik Sarfaraz Nawaz, Salini Scaria Joy, Teena P. George & Khalid Siddiqui

  2. University Diabetes Center, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Yousuf Al Farsi

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  1. Shaik Sarfaraz Nawaz
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  2. Salini Scaria Joy
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  5. Khalid Siddiqui
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Correspondence to Khalid Siddiqui.

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The study has been carried out under compliance of ethical issues. The study was approved by the institutional Review Committee of College of Medicine, King Saud University Riyadh. We used the STROBE case–control checklist for writing our report.

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Nawaz, S.S., Joy, S.S., Al Farsi, Y. et al. Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case–control study. Mol Biol Rep 46, 1239–1246 (2019). https://doi.org/10.1007/s11033-019-04592-2

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