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Changes in the expression level of IL-17A and p53-fibrinolytic system in smokers with or without COPD

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Abstract

COPD is a chronic airway inflammatory disease characterized mainly by neutrophil airway infiltrations. The neutrophil airway inflammation is mainly mediated through a key player like the pro-inflammatory cytokine IL-17A which is involved in the modulation of p53-fibrinolytic system. This study was undertaken to examine the molecular changes for the expressions of IL-17A and p53-fibrinolytic system in smokers with or without COPD. Blood and serum samples were collected from ten patients of smokers having COPD and ten samples from smokers without COPD and ten healthy control subjects. Western blot analyses were performed to evaluate the expressions of IL-17A, p53 and PAI-1. Apoptosis was assessed by immunoblot for cleaved caspase-3. In addition, FEV% was also determined of these patients. qRT-PCR was done to detect the gene expression study from the blood samples on p53-fibrinolytic components. A significant difference was found in the expression levels of IL-17A in smokers with COPD patient when compared to smokers without COPD and the control subjects. Similarly the smokers with COPD showed significant increase in the fibrinolytic component PAI-1 as well as in expression levels of p53 when compared to smokers without COPD and normal subjects. Increased cleaved caspase-3 may also promote apoptosis.The expression pattern of the IL-17A in chronic obstructive pulmonary distress syndrome samples was increased as compared of those of normal samples, and their main role in the regulation of and p53-fibrinolytic system makes these components as a predictive prominent component in smokers with COPD.

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Funding

This research work received funding from Yenepoya University Seed Grant No. 050-2015.

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Authors and Affiliations

  1. Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, Karnataka, 575 018, India

    Mahesh Manjunath Gouda, Sadiya Bi Shaikh & Yashodhar Bhandary

  2. Department of Pulmonary Medicine, Yenepoya Medical College (Deemed to be University), Deralakatte, Mangalore, Karnataka, 575018, India

    Deepu Chengappa & Irfan Kandhal

  3. Department of Anatomy, Yenepoya Medical College (Deemed to be University), Deralakatte, Mangalore, Karnataka, 575018, India

    Ashwini Shetty

Authors
  1. Mahesh Manjunath Gouda
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  2. Sadiya Bi Shaikh
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  3. Deepu Chengappa
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  4. Irfan Kandhal
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  5. Ashwini Shetty
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  6. Yashodhar Bhandary
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Correspondence to Yashodhar Bhandary.

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Gouda, M.M., Shaikh, S.B., Chengappa, D. et al. Changes in the expression level of IL-17A and p53-fibrinolytic system in smokers with or without COPD. Mol Biol Rep 45, 2835–2841 (2018). https://doi.org/10.1007/s11033-018-4398-y

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  • DOI: https://doi.org/10.1007/s11033-018-4398-y

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