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Black tea affects obesity by reducing nutrient intake and activating AMP-activated protein kinase in mice

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Abstract

The effects of certain tea components on the prevention of obesity in humans have been reported recently. However, whether Yinghong NO. 9 black tea consumption has beneficial effects on obesity are not known. Here, we obtained a Yinghong NO. 9 black tea infusion (Y9 BTI) and examined the anti-obesity effects of its oral administration. ICR mice were fed a standard diet supplemented with Y9 BTI at 0.5, 1.0, or 2.0 g/kg body weight for two weeks, and the body weight were recorded. HE staining was used to evaluate the effect of Y9 BTI on mice liver. Western blot analysis was used to detect the expression levels of related proteins in the mice liver and adipose. We found that the body weights of the mice in the control group were significantly higher than those of the mice in the middle and high dose groups. The results of western blot showed that Y9 BTI up-regulated the expression of liver kinase B1 (LKB1) and adenosine monophosphate-activated protein kinase (AMPK) and also increased in AMPK phosphorylation (p-AMPK) and LKB1 phosphorylation (p-LKB1). Y9 BTI significantly down-regulated Fas Cell Surface Death Receptor(FAS) and activated the phosphorylation of acetyl-CoA carboxylase (ACC). Furthermore, Y9 BTI (2.0 g/kg BW) down-regulated the expression of three factors (IL-1β, Cox-2, and iNOS). Altogether, Y9 BTI supplementation reduced the feed intake of mice and may prevent obesity by inhibiting lipid absorption. These results suggest that Y9 BTI may regulate adipogenic processes through the LKB1/AMPK pathway.

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Abbreviations

Y9 BTI:

Yinghong NO. 9 black tea infusion

ICR:

Institute of Cancer Research

LKB1:

Liver kinase B1

AMPK:

Adenosine monophosphate-activated protein kinase

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Acknowledgements

This work was financially supported by the Fund for Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization (No. 2008A060301004), the Construction of industrial technology innovation platform for black tea of Guangdong big leaves species, the earmarked fund for Modern Agro-industry Technology Research System (No. 2016LM2151), the Science and Technology Planning Project of Guangdong Province (Nos. 2016B090918118 and 2017A070702004), and the President Foundation of Guangdong Academy of Agricultural Sciences (Grant Nos. 201534 and 201720).

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  1. Shunshun Pan and Xuming Deng contributed equally to this work.

Authors and Affiliations

  1. Department of Tea Science, College of Horticulture, South China Agricultural University, No. 483, Wushan Road, Tianhe District, Guangzhou, 510642, People’s Republic of China

    Shunshun Pan, Xuming Deng, Lingzhi Zhang & Yahui Huang

  2. Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, 510640, People’s Republic of China

    Shunshun Pan, Shili Sun, Xingfei Lai, Lingli Sun, Qiuhua Li & Limin Xiang

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  1. Shunshun Pan
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Contributions

The authors’ responsibilities were as follows—SP, LZ and YH conceived and designed the experiments. SP, XD and SS performed the experiments. XD and XL analyzed the data. XL and LS contributed reagents and participated in animal anatomy. QL wrote the paper, and LX, LZ and YH critically revised the manuscript. All authors approved the final version of the manuscript.

Corresponding authors

Correspondence to Shili Sun, Lingzhi Zhang or Yahui Huang.

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Ethical approval

IACUC: Tea Research Institute, Guangdong Academy of Agricultural Sciences Institutional Review Board Approval of Experimental Animals, an approval number is Tea (2015) No. 004. In strict accordance with the promulgated in Guangdong Province, “Guangdong Province Experimental Animal Management Regulations,” the implementation of the use of CO2 inhalation after the injection of carotid artery blood to death, and then collected the relevant tissue samples.

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Pan, S., Deng, X., Sun, S. et al. Black tea affects obesity by reducing nutrient intake and activating AMP-activated protein kinase in mice. Mol Biol Rep 45, 689–697 (2018). https://doi.org/10.1007/s11033-018-4205-9

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