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IL-17A suppresses and curcumin up-regulates Akt expression upon bleomycin exposure

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Abstract

Pro-inflammatory cytokine IL-17A modulates the expression of Akt in bleomycin (BLM) administered alveolar basal epithelial cells, the mechanism behind which remains unclear. This investigation was carried out to assess IL-17A mediated down-regulation of Akt expression and the pivotal role of curcumin as a regulatory molecule. Alveolar basal epithelial cells were treated with BLM and IL-17A and curcumin was administered as an intervention to regulate the BLM-induced oxidative damage. Cell proliferation was evaluated by clonogenic assay. Akt phosphorylation and total Akt expressions were studied using western blot analysis. Cell proliferation reduced upon treatment with BLM and this phenomenon was reversed in cells upon administration with curcumin. Administrations of BLM and IL-17A to the alveolar basal epithelial cells showed significant down-regulation of Akt expression which was reversed by treatment with curcumin. BLM and IL-17A mediated inflammation was intervened effectively with curcumin. Results of this study suggest the probable use of curcumin as an anti-inflammatory therapeutic for lung injury.

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Acknowledgements

Authors would like to acknowledge the financial support rendered by Yenepoya University.

Funding

This work is supported by funding support from Indian Council of Medical Research (Grant No: 2015-1235) and research grants from Yenepoya University.

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Authors and Affiliations

  1. Yenepoya Research Centre, Yenepoya University, Deralakatte, Mangalore, Karnataka, 575 018, India

    Mahesh Manjunath Gouda, Ashwini Prabhu & Yashodhar Prabhakar Bhandary

Authors
  1. Mahesh Manjunath Gouda
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  2. Ashwini Prabhu
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  3. Yashodhar Prabhakar Bhandary
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Contributions

Conceived the idea and designed the experiments: YPB. Performed the experiments: MMG, AP. Data analysis and representation: MMG, AP.

Corresponding author

Correspondence to Yashodhar Prabhakar Bhandary.

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The authors declare that they have no conflict of interest.

Ethical approval

This is an in vitro study using immortalized cell lines. Ethical clearance is not applicable.

Additional information

Mahesh Manjunath Gouda and Ashwini Prabhu are equal first authors.

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Gouda, M.M., Prabhu, A. & Bhandary, Y.P. IL-17A suppresses and curcumin up-regulates Akt expression upon bleomycin exposure. Mol Biol Rep 45, 645–650 (2018). https://doi.org/10.1007/s11033-018-4199-3

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  • DOI: https://doi.org/10.1007/s11033-018-4199-3

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