Skip to main content
SpringerLink
Log in

Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench

  • Short Communication
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

DNA binding with one finger (Dof) proteins represent a family of plant specific transcription factors associated with diverse biological processes, such as seed maturation and germination, phytohormone and light mediated regulation, and plant responses to biotic and abiotic stresses. In present study, a total of 21 Dof genes from Sorghum bicolor were cloned, sequenced and in silico characterized for homology search, revealing their identity to Dof like proteins. The expression profiling of SbDof genes using quantitative RT-PCR in different tissue types and also under drought and salt stresses was attempted. The SbDof genes displayed differential expression either in their transcript abundance or in their expression patterns under normal growth condition. Two of the SbDof genes namely SbDof8 and SbDof12 showed comparatively high level of transcript abundance in all the tissue types tested; whereas some of the SbDof genes showed a distinct tissue specific expression pattern. Further a total of 13 SbDof genes showed differential expression when subjected to either of the abiotic stress i.e. drought or salinity. Three of the SbDof genes namely SbDof12, SbDof19 and SbDof24 were found to be up-regulated in response to drought and salt stress. Comparative analysis of SbDof genes expression revealed existence of a complex transcriptional and functional diversity across plant growth and developmental stages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Yanagisawa S (2002) The DOF family of plant transcription factors. Trends Plant Sci 7:555–560

    Article  CAS  PubMed  Google Scholar 

  2. Yanagisawa S (2004) DOF domain proteins: plant-specific transcription factors associated with diverse phenomena unique to plants. Plant Cell Physiol 45:386–391

    Article  CAS  PubMed  Google Scholar 

  3. Yanagisawa S (1995) A novel DNA-binding domain that may form a single zinc finger motif. Nucleic Acids Res 23:3403–3410

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Yanagisawa S, Schmidt RJ (1999) Diversity and similarity among recognition sequences of Dof transcription factors. Plant J 17:209–214

    Article  CAS  PubMed  Google Scholar 

  5. Gupta S, Malviya N, Kushwaha H, Nasim J, Bisht NC, Singh VK, Yadav D (2015) Insights into structural and functional diversity of Dof (DNA binding with one finger) transcription factor. Planta 241:549–562

    Article  CAS  PubMed  Google Scholar 

  6. Corrales AR, Nebauer SG, Carrillo L, Fernández-Nohales P, Marqués J, Renau-Morata B, Medina J (2014) Characterization of tomato cycling Dof factors reveals conserved and new functions in the control of flowering time and abiotic stress responses. J Exp Bot 65:995–1012

    Article  CAS  PubMed  Google Scholar 

  7. Moreno-Risueno MA, Martinez M, Vicente-Carbajosa J, Carbonero P (2007) The family of DOF transcription factors: from green unicellular algae to vascular plants. Mol Genet Gen 277:379–390

    Article  CAS  Google Scholar 

  8. Kushwaha H, Gupta S, Singh VK, Rastogi S, Yadav D (2011) Genome wide identification of Dof transcription factor gene family in Sorghum and its comparative phylogenetic analysis with rice and Arabidopsis. Mol Biol Rep 38:5037–5053

    Article  CAS  PubMed  Google Scholar 

  9. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  10. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Burland TG (2000) DNASTAR’s Lasergene sequence analysis software. Methods Mol Biol 132:71–91

    CAS  PubMed  Google Scholar 

  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  13. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  14. Kuriakosea B, Aruna V, Gnanamanickama SS, Thomas G (2009) Tissue-specific expression in transgenic rice and Arabidopsis thaliana plants of GUS gene driven by the 50 regulatory sequences of an anther specific rice gene YY2. Plant Sci 177:390–397

    Article  Google Scholar 

  15. Mena M, Vicente-Carbajosa J, Schmidt RJ, Carbonero P (1998) An endosperm-specific DOF protein from barley, highly conserved in wheat, binds to and activates transcription from the prolamin-box of a native B-hordein promoter in barley endosperm. Plant J 16:53–62

    Article  CAS  PubMed  Google Scholar 

  16. Rueda-Romero P, Barrero-Sicilia C, mez-Cadenas AG, Carbonero P, ate-Sa´ nchez LO (2012) Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14. J Exp Bot 63:1937–1949

    Article  CAS  PubMed  Google Scholar 

  17. Kim HS, Kim SJ, Abbasi N, Bressan RA, Yun DJ, Yoo SD (2010) The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis. Plant J 64:524–535

    Article  CAS  PubMed  Google Scholar 

  18. Guo Y, Qin G, Gu H, Qu LJ (2009) Dof5.6/HCA2, a Dof transcription factor gene, regulates interfascicular cambium formation and vascular tissue development in Arabidopsis. Plant Cell 21:3518–3534

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Li D, Yang C, Li X, Gan Q, Zhao X, Zhu L (2009) Functional characterization of rice OsDof12. Planta 229:1159–1169

    Article  CAS  PubMed  Google Scholar 

  20. Jiang Y, Deyholos MK (2006) Comprehensive transcriptional profiling of NaCl stressed Arabidopsis roots reveals novel classes of responsive genes. BMC Plant Biol 6:25

    Article  PubMed  PubMed Central  Google Scholar 

  21. Richardt S, Timmerhaus G, Lang D, Qudeimat E, Correˆa LGG, Reski R, Rensing SA, Frank W (2010) Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signaling. Plant Mol Biol 72:27–45

    Article  CAS  PubMed  Google Scholar 

  22. Shaw LM, McIntyre CL, Gresshoff PM, Xue GP (2009) Members of the Dof transcription factor family in Triticum aestivum are associated with light-mediated gene regulation. Funct Integr Genom 9:485–498

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge Head, Department of Biotechnology for providing infrastructural facilities. Prof. A. K. Tyagi, Director National Institute for Plant Genome Research, New Delhi is thankfully acknowledged for allowing SG to perform gene expression work. SG and GCA acknowledge Council of Scientific and Industrial Research for Senior Research fellowship. The author wishes to acknowledge Prof. P.K Shrotria, Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand for providing the seeds of sorghum.

Author information

Authors and Affiliations

  1. Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh, 273 009, India

    Shubhra Gupta, Neha Malviya & Dinesh Yadav

  2. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110 067, India

    Gulab C. Arya & Naveen C. Bisht

Authors
  1. Shubhra Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gulab C. Arya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Neha Malviya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Naveen C. Bisht
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dinesh Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dinesh Yadav.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 25 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gupta, S., Arya, G.C., Malviya, N. et al. Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench. Mol Biol Rep 43, 767–774 (2016). https://doi.org/10.1007/s11033-016-4019-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-016-4019-6

Keywords

Search

Navigation