Abstract
Isothiazolone biocides (such as Kathon) are widely used in a variety of industrial and domestic applications. However, the mechanisms through which bacteria develop resistance to these biocides are not completely clear. A better understanding of these mechanisms can contribute to optimal use of these biocides. In this study, transcription profiles of a Kathon-resistant strain of Pseudomonas aeruginosa (Pa-R) and the wild-type strain were determined using RNA sequencing (RNA-Seq) with the Illumina HiSeq 2000 platform. RNA-Seq generated 18,657,896 sequence reads aligned to 7093 genes. In all, 1550 differently expressed genes (DEGs, log2 ratio ≥1, false discovery rate (FDR) ≤0.001) were identified, of which 482 were up-regulated and 1068 were down-regulated. Most Kathon-induced genes were involved in metabolic and cellular processes. DEGs significantly enriched nitrogen metabolism and oxidative phosphorylation pathways. In addition, Pa-R showed cross-resistance to triclosan and ciprofloxacin and showed repressed pyocyanin production. These results may improve our understanding of the resistance mechanisms of P. aeruginosa against isothiazolones, and provide insight into the development of more efficient isothiazolones.
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Acknowledgments
Financial supports were provided by the National Natural Science Foundation of China (No. 31500036), Natural Science Foundation of Guangdong Province (No. 2015A030313713), Cooperation Projects of Foshan City and Chinese Academy (No. 2012HY100115) and Jieyang Cooperation Projects of Industry-University-Research (No. 201429).
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Zhou, G., Shi, Qs., Huang, Xm. et al. Comparison of transcriptomes of wild-type and isothiazolone-resistant Pseudomonas aeruginosa by using RNA-seq. Mol Biol Rep 43, 527–540 (2016). https://doi.org/10.1007/s11033-016-3978-y
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DOI: https://doi.org/10.1007/s11033-016-3978-y