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Transcriptome-wide identification of the genes responding to replanting disease in Rehmannia glutinosa L. roots

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Abstract

The development of the medicinal plant Rehmannia glutinosa L. are severely declined when are replanted on the soil of the preceding crops being themselves. The biological basis of this so called “replanting disease” is unknown. Here, we have exploited the parallel sequencing capacity of both RNA-seq and DGE technology to ascertain what genes are responsive to the replanting disease in roots of R. glutinosa. RNA-seq analysis generated 99,708 non-redundant consensus sequences from the roots of the first year (R1) and the second year (R2) replanted R. glutinosa plants. From this set, a total of 48,616 transcripts containing a complete or partial encoding region was identified. Based on this resource, two DGE tag libraries were established to capture the transcriptome differences between the R1 and R2 libraries. Finally, a set of 2,817 (1,676 up- and 1,141 down-regulated) differentially transcribed genes was screened, and 114 most strongly differentially transcribed genes were identified by DGE analysis between first year and replanted plants. Furthermore, a more detailed examination of 16 selected candidates was carried out by qRT-PCR. The indication was that replanting could promote Ca2+ signal transduction and ethylene synthesis, resulting in forming of the replanting disease. We analyzed the biomass indexes of replanted R. glutinosa roots by irrigating Ca2+ signal blockers. The results suggested that the alleviation of the disease impairment could be the decrease of Ca2+ signal transduction. This study provided a global survey of the root transcriptome in replanted R. glutinosa roots at the tuberous root expansion stage. As a result, a number of candidate genes underlying the replanting disease have been identified.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Nos. 31271674, 81274022 and 81403037), the Science and Technology Research Key Project of Henan Educational Committee (No. 13A180160) and High-level Personnel Scientific Research Start-up Foundation of Henan University of Technology (No. 150512).

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Authors and Affiliations

  1. College of Bioengineering, Henan University of Technology, Zhengzhou, 450001, China

    Yan Hui Yang

  2. College of Life Sciences, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, Henan, China

    Xin Yu Li & Xin Jian Chen

  3. College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Ming Jie Li, Wen Xiong Lin & Zhong Yi Zhang

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  1. Yan Hui Yang
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Correspondence to Xin Jian Chen or Zhong Yi Zhang.

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Yang, Y.H., Li, M.J., Li, X.Y. et al. Transcriptome-wide identification of the genes responding to replanting disease in Rehmannia glutinosa L. roots. Mol Biol Rep 42, 881–892 (2015). https://doi.org/10.1007/s11033-014-3825-y

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